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Gräwe, Alexander ; Merkx, Maarten ; Stein, Viktor (2022):
iFLinkC-X: A Scalable Framework to Assemble Bespoke Genetically Encoded Co-polymeric Linkers of Variable Lengths and Amino Acid Composition.
In: Bioconjugate chemistry, 33 (7), pp. 1415-1421. ISSN 1520-4812,
DOI: 10.1021/acs.bioconjchem.2c00250,
[Article]
Weber, Wadim ; Roeder, Markus ; Probanowski, Tobias ; Yang, Jie ; Abujubara, Helal ; Koeppl, Heinz ; Tietze, Alesia ; Stein, Viktor (2022):
Functional Nanopore Screen: A Versatile High-Throughput Assay to Study and Engineer Protein Nanopores in Escherichia coli.
In: ACS synthetic biology, 11 (6), pp. 2070-2079. ISSN 2161-5063,
DOI: 10.1021/acssynbio.1c00635,
[Article]
Duznovic, Ivana ; Gräwe, Alexander ; Weber, Wadim ; Müller, Lena K. ; Ali, Mubarak ; Ensinger, Wolfgang ; Tietze, Alesia ; Stein, Viktor (2021):
Ultrasensitive and Selective Protein Recognition with Nanobody-Functionalized Synthetic Nanopores.
In: Small (Weinheim an der Bergstrasse, Germany), ISSN 1613-6829,
DOI: 10.1002/smll.202101066,
[Article]
Gräwe, Alexander ; Ranglack, Jan ; Weyrich, Anastasia ; Stein, Viktor (2021):
Synthetic protein switches: Combinatorial linker engineering with iFLinkC.
In: Methods in enzymology, 647, pp. 231-255. ISSN 1557-7988,
DOI: 10.1016/bs.mie.2020.09.009,
[Article]
Gräwe, Alexander ; Stein, Viktor (2020):
Linker engineering in the context of synthetic protein switches and sensors.
In: Trends in biotechnology, ISSN 1879-3096,
DOI: 10.1016/j.tibtech.2020.11.007,
[Article]
Müller, Lena K. ; Duznovic, Ivana ; Tietze, Daniel ; Weber, Wadim ; Ali, Mubarak ; Stein, Viktor ; Ensinger, Wolfgang ; Tietze, Alesia A. (2020):
Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor.
In: Chemistry – A European Journal, 26 (39), pp. 8511-8517. Wiley-VCH, ISSN 1521-3765,
DOI: 10.1002/chem.202001160,
[Article]
Gräwe, Alexander ; Ranglack, Jan ; Weyrich, Anastasia ; Stein, Viktor (2020):
iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains.
In: Nucleic acids research, 48 (4), pp. e24. ISSN 1362-4962,
DOI: 10.1093/nar/gkz1210,
[Article]
Gräwe, Alexander ; Ranglack, Jan ; Weber, Wadim ; Stein, Viktor (2019):
Engineering artificial signalling functions with proteases.
In: Current opinion in biotechnology, 63, pp. 1-7. ISSN 1879-0429,
DOI: 10.1016/j.copbio.2019.09.017,
[Article]
Khoury, Mario El ; Winterstein, Tobias ; Weber, Wadim ; Stein, Viktor ; Schlaak, Helmut F. ; Thiel, Gerhard (2019):
Photolithographic Fabrication of Micro Apertures in Dry Film Polymer Sheets for Channel Recordings in Planar Lipid Bilayers.
In: The Journal of membrane biology, 252 (2/3), pp. 173-182. ISSN 1432-1424,
DOI: 10.1007/s00232-019-00062-9,
[Article]
Stein, Viktor ; Alexandrov, Kirill (2017):
Engineering and Characterizing Synthetic Protease Sensors and Switches.
In: Methods in molecular biology (Clifton, N.J.), 1596, pp. 197-218. ISSN 1940-6029,
[Article]
Stein, Viktor (2017):
Synthetic Protein Switches: Theoretical and Experimental Considerations.
In: Methods in molecular biology (Clifton, N.J.), 1596, pp. 3-25. ISSN 1940-6029,
[Article]
Guo, Zhong ; Murphy, Lindy ; Stein, Viktor ; Johnston, Wayne A. ; Alcala-Perez, Siro ; Alexandrov, Kirill (2016):
Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform.
In: Journal of the American Chemical Society, 138 (32), pp. 10108-11. ISSN 1520-5126,
[Article]
Guo, Zhong ; Johnston, Wayne A. ; Stein, Viktor ; Kalimuthu, Palraj ; Perez-Alcala, Siro ; Bernhardt, Paul V. ; Alexandrov, Kirill (2016):
Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca(2+) sensor.
In: Chemical communications (Cambridge, England), 52 (3), pp. 485-8. ISSN 1364-548X,
[Article]
Cui, Zhenling ; Stein, Viktor ; Tnimov, Zakir ; Mureev, Sergey ; Alexandrov, Kirill (2015):
Semisynthetic tRNA complement mediates in vitro protein synthesis.
In: Journal of the American Chemical Society, 137 (13), pp. 4404-13. ISSN 1520-5126,
[Article]
Stein, Viktor ; Alexandrov, Kirill (2015):
Synthetic protein switches: design principles and applications.
In: Trends in biotechnology, 33 (2), pp. 101-10. ISSN 1879-3096,
[Article]
Stein, Viktor ; Kubala, Marta H. ; Steen, Jason ; Grimmond, Sean M. ; Alexandrov, Kirill (2015):
Towards the systematic mapping and engineering of the protein prenylation machinery in Saccharomyces cerevisiae.
In: PloS one, 10 (3), pp. e0120716. ISSN 1932-6203,
[Article]
Stein, Viktor ; Alexandrov, Kirill (2014):
Protease-based synthetic sensing and signal amplification.
In: Proceedings of the National Academy of Sciences of the United States of America, 111 (45), pp. 15934-9. ISSN 1091-6490,
[Article]
Stein, Viktor ; Kaltenbach, Miriam ; Hollfelder, Florian (2012):
Assembling linear DNA templates for in vitro transcription and translation.
In: Methods in molecular biology (Clifton, N.J.), 815, pp. 67-78. ISSN 1940-6029,
[Article]
Kaltenbach, Miriam ; Stein, Viktor ; Hollfelder, Florian (2011):
SNAP dendrimers: multivalent protein display on dendrimer-like DNA for directed evolution.
In: Chembiochem : a European journal of chemical biology, 12 (14), pp. 2208-16. ISSN 1439-7633,
[Article]
Schaerli, Yolanda ; Stein, Viktor ; Spiering, Michelle M. ; Benkovic, Stephen J. ; Abell, Chris ; Hollfelder, Florian (2010):
Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification.
In: Nucleic acids research, 38 (22), pp. e201. ISSN 1362-4962,
[Article]
Villiers, B. R. M. ; Stein, Viktor ; Hollfelder, F. (2010):
USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction.
In: Protein engineering, design & selection : PEDS, 23 (1), pp. 1-8. ISSN 1741-0134,
[Article]
Schaerli, Yolanda ; Wootton, Robert C. ; Robinson, Tom ; Stein, Viktor ; Dunsby, Christopher ; Neil, Mark A. A. ; French, Paul M. W. ; Demello, Andrew J. ; Abell, Chris ; Hollfelder, Florian (2009):
Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets.
In: Analytical chemistry, 81 (1), pp. 302-6. ISSN 1520-6882,
[Article]
Smith, James ; Stein, Viktor (2009):
SPORCalc: A development of a database analysis that provides putative metabolic enzyme reactions for ligand-based drug design.
In: Computational biology and chemistry, 33 (2), pp. 149-59. ISSN 1476-928X,
[Article]
Stein, Viktor ; Hollfelder, Florian (2009):
An efficient method to assemble linear DNA templates for in vitro screening and selection systems.
In: Nucleic acids research, 37 (18), pp. e122. ISSN 1362-4962,
[Article]
Boyer, Scott ; Arnby, Catrin Hasselgren ; Carlsson, Lars ; Smith, James ; Stein, Viktor ; Glen, Robert C. (2007):
Reaction site mapping of xenobiotic biotransformations.
In: Journal of chemical information and modeling, 47 (2), pp. 583-90. ISSN 1549-9596,
[Article]
Stein, Viktor ; Sielaff, India ; Johnsson, Kai ; Hollfelder, Florian (2007):
A covalent chemical genotype-phenotype linkage for in vitro protein evolution.
In: Chembiochem : a European journal of chemical biology, 8 (18), pp. 2191-4. ISSN 1439-7633,
[Article]
Siskos, Alexandros P. ; Baerga-Ortiz, Abel ; Bali, Shilpa ; Stein, Viktor ; Mamdani, Hassan ; Spiteller, Dieter ; Popovic, Bojana ; Spencer, Jonathan B. ; Staunton, James ; Weissman, Kira J. ; Leadlay, Peter F. (2005):
Molecular basis of Celmer's rules: stereochemistry of catalysis by isolated ketoreductase domains from modular polyketide synthases.
In: Chemistry & biology, 12 (10), pp. 1145-53. ISSN 1074-5521,
[Article]
Leemhuis, Hans ; Stein, Viktor ; Griffiths, Andrew D. ; Hollfelder, Florian (2005):
New genotype-phenotype linkages for directed evolution of functional proteins.
In: Current opinion in structural biology, 15 (4), pp. 472-8. ISSN 0959-440X,
[Article]