Stein, Viktor ; Kaltenbach, Miriam ; Hollfelder, Florian (2012)
Assembling linear DNA templates for in vitro transcription and translation.
In: Methods in molecular biology (Clifton, N.J.), 815
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Cell-free expression systems provide straightforward access from genes to the corresponding proteins, involving fewer handling steps than in vivo procedures. A quick procedure to assemble a gene of interest into a linear DNA template together with 3'- and 5'-untranslated regions using a coupled uracil-excision-ligation strategy based on USER Enzyme and T4 DNA ligase. This methodology will be useful for repeated cycles of expression and in vitro selection, in which gene libraries are repeatedly assembled and their products and templates regenerated.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Stein, Viktor ; Kaltenbach, Miriam ; Hollfelder, Florian |
| Art des Eintrags: | Bibliographie |
| Titel: | Assembling linear DNA templates for in vitro transcription and translation. |
| Sprache: | Englisch |
| Publikationsjahr: | 2012 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods in molecular biology (Clifton, N.J.) |
| Jahrgang/Volume einer Zeitschrift: | 815 |
| Kurzbeschreibung (Abstract): | Cell-free expression systems provide straightforward access from genes to the corresponding proteins, involving fewer handling steps than in vivo procedures. A quick procedure to assemble a gene of interest into a linear DNA template together with 3'- and 5'-untranslated regions using a coupled uracil-excision-ligation strategy based on USER Enzyme and T4 DNA ligase. This methodology will be useful for repeated cycles of expression and in vitro selection, in which gene libraries are repeatedly assembled and their products and templates regenerated. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Protein Engineering of Ion Conducting Nanopores |
| Hinterlegungsdatum: | 14 Nov 2016 11:35 |
| Letzte Änderung: | 14 Nov 2016 11:35 |
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