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Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor

Müller, Lena K. and Duznovic, Ivana and Tietze, Daniel and Weber, Wadim and Ali, Mubarak and Stein, Viktor and Ensinger, Wolfgang and Tietze, Alesia A. (2020):
Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor.
In: Chemistry – A European Journal, 26 (39), pp. 8511-8517. Wiley-VCH, ISSN 1521-3765,
DOI: 10.1002/chem.202001160,
[Article]

Abstract

We report a nanopore-based Cu(II) sensing system which allows for an ultrasensitive and selective detection of Cu(II) with the possibility for a broad range of applications, i.e. medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu(II) ions in the presence of Ni(II) and Zn(II) and was crafted into ion track-etched nanopores. Upon Cu(II) binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II) in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II) allowing to sense femtomolar Cu(II) concentrations in human urine mimics. Washing with EDTA fully restores the Cu(II)-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu(II)-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II) levels in patients with copper dysfunctional homeostasis.

Item Type: Article
Erschienen: 2020
Creators: Müller, Lena K. and Duznovic, Ivana and Tietze, Daniel and Weber, Wadim and Ali, Mubarak and Stein, Viktor and Ensinger, Wolfgang and Tietze, Alesia A.
Title: Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor
Language: English
Abstract:

We report a nanopore-based Cu(II) sensing system which allows for an ultrasensitive and selective detection of Cu(II) with the possibility for a broad range of applications, i.e. medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu(II) ions in the presence of Ni(II) and Zn(II) and was crafted into ion track-etched nanopores. Upon Cu(II) binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II) in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II) allowing to sense femtomolar Cu(II) concentrations in human urine mimics. Washing with EDTA fully restores the Cu(II)-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu(II)-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II) levels in patients with copper dysfunctional homeostasis.

Journal or Publication Title: Chemistry – A European Journal
Journal volume: 26
Number: 39
Publisher: Wiley-VCH
Uncontrolled Keywords: Liebig Fellowship of the Fonds der Chemischen Industrie, LOEWE project iNAPO - Ministry of Higher education, Research and the Arts (HMWK) of the Hessen state, Knut & Alice Wallenberg Foundation, ATCUN, copper, fluorescence, nanopores, sensors
Divisions: 10 Department of Biology
10 Department of Biology > Protein Engineering of Ion Conducting Nanopores
11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Material Analytics
Date Deposited: 25 Mar 2020 07:46
DOI: 10.1002/chem.202001160
Identification Number: pmid:32196774
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