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Synthetic Protein Switches: Theoretical and Experimental Considerations.

Stein, Viktor (2017):
Synthetic Protein Switches: Theoretical and Experimental Considerations.
In: Methods in molecular biology (Clifton, N.J.), pp. 3-25, 1596, ISSN 1940-6029, [Article]

Abstract

Synthetic protein switches with tailored response functions are finding increasing applications as tools in basic research and biotechnology. With a number of successful design strategies emerging, the construction of synthetic protein switches still frequently necessitates an integrated approach that combines detailed biochemical and biophysical characterization in combination with high-throughput screening to construct tailored synthetic protein switches. This is increasingly complemented by computational strategies that aim to reduce the need for costly empirical optimization and thus facilitate the protein design process. Successful computational design approaches range from analyzing phylogenetic data to infer useful structural, biophysical, and biochemical information to modeling the structure and function of proteins ab initio. The following chapter provides an overview over the theoretical considerations and experimental approaches that have been successful applied in the construction of synthetic protein switches.

Item Type: Article
Erschienen: 2017
Creators: Stein, Viktor
Title: Synthetic Protein Switches: Theoretical and Experimental Considerations.
Language: English
Abstract:

Synthetic protein switches with tailored response functions are finding increasing applications as tools in basic research and biotechnology. With a number of successful design strategies emerging, the construction of synthetic protein switches still frequently necessitates an integrated approach that combines detailed biochemical and biophysical characterization in combination with high-throughput screening to construct tailored synthetic protein switches. This is increasingly complemented by computational strategies that aim to reduce the need for costly empirical optimization and thus facilitate the protein design process. Successful computational design approaches range from analyzing phylogenetic data to infer useful structural, biophysical, and biochemical information to modeling the structure and function of proteins ab initio. The following chapter provides an overview over the theoretical considerations and experimental approaches that have been successful applied in the construction of synthetic protein switches.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Volume: 1596
Divisions: 10 Department of Biology
10 Department of Biology > Protein Engineering of Ion Conducting Nanopores
Date Deposited: 23 Mar 2017 08:02
Identification Number: pmid:28293877
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