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New genotype-phenotype linkages for directed evolution of functional proteins.

Leemhuis, Hans and Stein, Viktor and Griffiths, Andrew D. and Hollfelder, Florian (2005):
New genotype-phenotype linkages for directed evolution of functional proteins.
In: Current opinion in structural biology, pp. 472-8, 15, (4), ISSN 0959-440X,
[Article]

Abstract

The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype. In vitro selections offer the potential to select from libraries with up to 10(10)-10(14) members, with fewer constraints than current cell-based selections. New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis.

Item Type: Article
Erschienen: 2005
Creators: Leemhuis, Hans and Stein, Viktor and Griffiths, Andrew D. and Hollfelder, Florian
Title: New genotype-phenotype linkages for directed evolution of functional proteins.
Language: English
Abstract:

The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype. In vitro selections offer the potential to select from libraries with up to 10(10)-10(14) members, with fewer constraints than current cell-based selections. New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis.

Journal or Publication Title: Current opinion in structural biology
Volume: 15
Number: 4
Divisions: 10 Department of Biology
10 Department of Biology > Protein Engineering of Ion Conducting Nanopores
Date Deposited: 14 Nov 2016 11:59
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