Guo, Zhong ; Johnston, Wayne A. ; Stein, Viktor ; Kalimuthu, Palraj ; Perez-Alcala, Siro ; Bernhardt, Paul V. ; Alexandrov, Kirill (2016)
Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca(2+) sensor.
In: Chemical communications (Cambridge, England), 52 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Electrochemical biosensors convert biological events to an electrical current. To date most electrochemical biosensors exploit activities of naturally occurring enzymes. Here we demonstrated that insertion of a calmodulin domain into the redox enzyme PQQ-glucose dehydrogenase resulted in a selective Ca(2+) biosensor that could be used to rapidly measure Ca(2+) concentrations in human biological fluids. We were able to convert a point-of-care glucometer into Ca(2+) monitor by refurbishing it with the developed biosensor. We propose that similar engineering strategies may be used to create highly specific electrochemical biosensors to other analytes. Compatibility with cheap and ubiquitous amperometric detectors is expected to accelerate progression of these biosensors into clinical applications.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2016 |
| Autor(en): | Guo, Zhong ; Johnston, Wayne A. ; Stein, Viktor ; Kalimuthu, Palraj ; Perez-Alcala, Siro ; Bernhardt, Paul V. ; Alexandrov, Kirill |
| Art des Eintrags: | Bibliographie |
| Titel: | Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca(2+) sensor. |
| Sprache: | Englisch |
| Publikationsjahr: | 2016 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Chemical communications (Cambridge, England) |
| Jahrgang/Volume einer Zeitschrift: | 52 |
| (Heft-)Nummer: | 3 |
| Kurzbeschreibung (Abstract): | Electrochemical biosensors convert biological events to an electrical current. To date most electrochemical biosensors exploit activities of naturally occurring enzymes. Here we demonstrated that insertion of a calmodulin domain into the redox enzyme PQQ-glucose dehydrogenase resulted in a selective Ca(2+) biosensor that could be used to rapidly measure Ca(2+) concentrations in human biological fluids. We were able to convert a point-of-care glucometer into Ca(2+) monitor by refurbishing it with the developed biosensor. We propose that similar engineering strategies may be used to create highly specific electrochemical biosensors to other analytes. Compatibility with cheap and ubiquitous amperometric detectors is expected to accelerate progression of these biosensors into clinical applications. |
| ID-Nummer: | pmid:26528736 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Protein Engineering of Ion Conducting Nanopores |
| Hinterlegungsdatum: | 14 Nov 2016 11:25 |
| Letzte Änderung: | 22 Nov 2016 11:18 |
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