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Engineering and Characterizing Synthetic Protease Sensors and Switches.

Stein, Viktor and Alexandrov, Kirill (2017):
Engineering and Characterizing Synthetic Protease Sensors and Switches.
In: Methods in molecular biology (Clifton, N.J.), pp. 197-218, 1596, ISSN 1940-6029, [Article]

Abstract

Proteases are finding an increasing number of applications as molecular tools and reporters in biotechnology and basic research. Proteases are also increasingly incorporated into synthetic genetic signaling circuits equipping cells with tailored new functions. In the majority of cases however, proteases are employed in constitutively active forms which limits their utility and application as molecular sensors. The following chapter provides a detailed experimental protocol for converting constitutively active proteases into regulated protease receptors. Such receptors can potentially sense, transduce, and amplify any molecular input, thereby opening up a range of new applications in basic research, biotechnology, and synthetic biology.

Item Type: Article
Erschienen: 2017
Creators: Stein, Viktor and Alexandrov, Kirill
Title: Engineering and Characterizing Synthetic Protease Sensors and Switches.
Language: English
Abstract:

Proteases are finding an increasing number of applications as molecular tools and reporters in biotechnology and basic research. Proteases are also increasingly incorporated into synthetic genetic signaling circuits equipping cells with tailored new functions. In the majority of cases however, proteases are employed in constitutively active forms which limits their utility and application as molecular sensors. The following chapter provides a detailed experimental protocol for converting constitutively active proteases into regulated protease receptors. Such receptors can potentially sense, transduce, and amplify any molecular input, thereby opening up a range of new applications in basic research, biotechnology, and synthetic biology.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Volume: 1596
Divisions: 10 Department of Biology
10 Department of Biology > Protein Engineering of Ion Conducting Nanopores
Date Deposited: 23 Mar 2017 08:01
Identification Number: pmid:28293889
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