Stein, Viktor ; Alexandrov, Kirill (2017)
Engineering and Characterizing Synthetic Protease Sensors and Switches.
In: Methods in molecular biology (Clifton, N.J.), 1596
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Proteases are finding an increasing number of applications as molecular tools and reporters in biotechnology and basic research. Proteases are also increasingly incorporated into synthetic genetic signaling circuits equipping cells with tailored new functions. In the majority of cases however, proteases are employed in constitutively active forms which limits their utility and application as molecular sensors. The following chapter provides a detailed experimental protocol for converting constitutively active proteases into regulated protease receptors. Such receptors can potentially sense, transduce, and amplify any molecular input, thereby opening up a range of new applications in basic research, biotechnology, and synthetic biology.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Stein, Viktor ; Alexandrov, Kirill |
| Art des Eintrags: | Bibliographie |
| Titel: | Engineering and Characterizing Synthetic Protease Sensors and Switches. |
| Sprache: | Englisch |
| Publikationsjahr: | 2017 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods in molecular biology (Clifton, N.J.) |
| Jahrgang/Volume einer Zeitschrift: | 1596 |
| Kurzbeschreibung (Abstract): | Proteases are finding an increasing number of applications as molecular tools and reporters in biotechnology and basic research. Proteases are also increasingly incorporated into synthetic genetic signaling circuits equipping cells with tailored new functions. In the majority of cases however, proteases are employed in constitutively active forms which limits their utility and application as molecular sensors. The following chapter provides a detailed experimental protocol for converting constitutively active proteases into regulated protease receptors. Such receptors can potentially sense, transduce, and amplify any molecular input, thereby opening up a range of new applications in basic research, biotechnology, and synthetic biology. |
| ID-Nummer: | pmid:28293889 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Protein Engineering of Ion Conducting Nanopores |
| Hinterlegungsdatum: | 23 Mär 2017 08:01 |
| Letzte Änderung: | 23 Mär 2017 08:01 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum