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Engineering artificial signalling functions with proteases.

Gräwe, Alexander ; Ranglack, Jan ; Weber, Wadim ; Stein, Viktor (2019)
Engineering artificial signalling functions with proteases.
In: Current opinion in biotechnology, 63
doi: 10.1016/j.copbio.2019.09.017
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Proteases have emerged as a promising class of enzymes to build post-translationally regulated signalling functions in diverse organisms and cell types ranging from simple prokaryotes to higher eukaryotes and in reconstituted systems in vitro. An expanding repertoire of proteases can now be readily configured to build tailored sensors, switches and transducers, and is increasingly facilitating the construction of complex sensory systems for a variety of biotechnological and biomedical applications. This is complemented by an increasing understanding of the fundamental design principles underlying biological signal processing at both protein-level and circuit-level that is now actively probed through synthesis. This review thus aims to summarize and analyse the most promising conceptual and experimental approaches that can be applied to build artificial signalling functions with proteases while highlighting advances, drawbacks and limitations.

Typ des Eintrags: Artikel
Erschienen: 2019
Autor(en): Gräwe, Alexander ; Ranglack, Jan ; Weber, Wadim ; Stein, Viktor
Art des Eintrags: Bibliographie
Titel: Engineering artificial signalling functions with proteases.
Sprache: Englisch
Publikationsjahr: 4 November 2019
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Current opinion in biotechnology
Jahrgang/Volume einer Zeitschrift: 63
DOI: 10.1016/j.copbio.2019.09.017
Kurzbeschreibung (Abstract):

Proteases have emerged as a promising class of enzymes to build post-translationally regulated signalling functions in diverse organisms and cell types ranging from simple prokaryotes to higher eukaryotes and in reconstituted systems in vitro. An expanding repertoire of proteases can now be readily configured to build tailored sensors, switches and transducers, and is increasingly facilitating the construction of complex sensory systems for a variety of biotechnological and biomedical applications. This is complemented by an increasing understanding of the fundamental design principles underlying biological signal processing at both protein-level and circuit-level that is now actively probed through synthesis. This review thus aims to summarize and analyse the most promising conceptual and experimental approaches that can be applied to build artificial signalling functions with proteases while highlighting advances, drawbacks and limitations.

ID-Nummer: pmid:31698156
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Protein Engineering of Ion Conducting Nanopores
Hinterlegungsdatum: 14 Nov 2019 08:47
Letzte Änderung: 14 Nov 2019 08:47
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