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Milking the cow: cattle-derived chimeric ultralong CDR-H3 antibodies and their engineered CDR-H3-only knobbody counterparts targeting epidermal growth factor receptor elicit potent NK cell-mediated cytotoxicity.
In: Frontiers in Immunology, 12
doi: 10.3389/fimmu.2021.742418
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Becker, Bastian ; Englert, Simon ; Schneider, Hendrik ; Yanakieva, Desislava ; Hofmann, Sarah ; Dombrowsky, Carolin ; Macarrón Palacios, Arturo ; Bitsch, Sebastian ; Elter, Adrian ; Meckel, Tobias ; Kugler, Benedikt ; Schirmacher, Anastasyia ; Avrutina, Olga ; Diederichsen, Ulf ; Kolmar, Harald (2021)
Multivalent dextran hybrids for efficient cytosolic delivery of biomolecular cargoes.
In: Journal of Peptide Science : the official Journal of the European Peptide Society, 27 (4)
doi: 10.1002/psc.3298
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Elter, Adrian ; Yanakieva, Desislava ; Fiebig, David ; Hallstein, Kerstin ; Becker, Stefan ; Betz, Ulrich ; Kolmar, Harald (2021)
Protease-Activation of Fc-Masked Therapeutic Antibodies to Alleviate Off-Tumor Cytotoxicity.
In: Frontiers in Immunology, 12
doi: 10.3389/fimmu.2021.715719
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Yanakieva, Desislava ; Elter, Adrian ; Bratsch, Jens ; Friedrich, Karlheinz ; Becker, Stefan ; Kolmar, Harald (2020)
FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells.
In: Scientific Reports, (10)
doi: 10.1038/S41598-020-66927-5
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Bogen, Jan P. ; Storka, Juliana ; Yanakieva, Desislava ; Fiebig, David ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2020)
Isolation of common light chain antibodies from immunized chickens using yeast biopanning and fluorescence‐activated cell sorting.
In: Biotechnology Journal : Systems and Synthetic Biology, Nanobiotech, Medicine, 16 (3)
doi: 10.1002/biot.202000240
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Yanakieva, Desislava (2020)
Ultra-High-Throughput Single-Cell Functional Screening by Combination of Microfluidics and GMD-FACS.
Technische Universität Darmstadt
doi: 10.25534/tuprints-00013464
Dissertation, Erstveröffentlichung

Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald (2019)
Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
In: Chemistry (Weinheim an der Bergstrasse, Germany), 25 (7)
doi: 10.1002/chem.201804889
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Könning, Doreen ; Rhiel, Laura ; Empting, Martin ; Grzeschik, Julius ; Sellmann, Carolin ; Schröter, Christian ; Zielonka, Stefan ; Dickgießer, Stephan ; Pirzer, Thomas ; Yanakieva, Desislava ; Becker, Stefan ; Kolmar, Harald (2017)
Semi-synthetic vNAR libraries screened against therapeutic antibodies primarily deliver anti-idiotypic binders.
In: Scientific Reports, 7 (1)
doi: 10.1038/s41598-017-10513-9
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