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Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.

Hilberg, Valentina and Avrutina, Olga and Ebenig, Aileen and Yanakieva, Desislava and Meckel, Tobias and Biesalski, Markus and Kolmar, Harald (2019):
Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
In: Chemistry (Weinheim an der Bergstrasse, Germany), pp. 1746-1751, 25, (7), ISSN 1521-3765, DOI: 10.1002/chem.201804889, [Online-Edition: https://doi.org/10.1002/chem.201804889],
[Article]

Abstract

Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.

Item Type: Article
Erschienen: 2019
Creators: Hilberg, Valentina and Avrutina, Olga and Ebenig, Aileen and Yanakieva, Desislava and Meckel, Tobias and Biesalski, Markus and Kolmar, Harald
Title: Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
Language: English
Abstract:

Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.

Journal or Publication Title: Chemistry (Weinheim an der Bergstrasse, Germany)
Volume: 25
Number: 7
Divisions: 10 Department of Biology
10 Department of Biology > Membrane Dynamics
Date Deposited: 13 Nov 2018 07:50
DOI: 10.1002/chem.201804889
Official URL: https://doi.org/10.1002/chem.201804889
Identification Number: pmid:30395364
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