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Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.

Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald :
Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
[Online-Edition: https://doi.org/10.1002/chem.201804889]
In: Chemistry (Weinheim an der Bergstrasse, Germany), 25 (7) S. 1746-1751. ISSN 1521-3765
[Artikel] , (2019)

Offizielle URL: https://doi.org/10.1002/chem.201804889

Kurzbeschreibung (Abstract)

Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.

Typ des Eintrags: Artikel
Erschienen: 2019
Autor(en): Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald
Titel: Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Chemistry (Weinheim an der Bergstrasse, Germany)
Band: 25
(Heft-)Nummer: 7
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Membrane Dynamics
Hinterlegungsdatum: 13 Nov 2018 07:50
DOI: 10.1002/chem.201804889
Offizielle URL: https://doi.org/10.1002/chem.201804889
ID-Nummer: pmid:30395364
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