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Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells.

Borcherds, Wade ; Theillet, François-Xavier ; Katzer, Andrea ; Finzel, Ana ; Mishall, Katie M. ; Powell, Anne T. ; Wu, Hongwei ; Manieri, Wanda ; Dieterich, Christoph ; Selenko, Philipp ; Loewer, Alexander ; Daughdrill, Gary W. :
Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells.
In: Nature chemical biology, 10 (12) pp. 1000-2. ISSN 1552-4469
[Artikel], (2014)

Kurzbeschreibung (Abstract)

Levels of residual structure in disordered interaction domains determine in vitro binding affinities, but whether they exert similar roles in cells is not known. Here, we show that increasing residual p53 helicity results in stronger Mdm2 binding, altered p53 dynamics, impaired target gene expression and failure to induce cell cycle arrest upon DNA damage. These results establish that residual structure is an important determinant of signaling fidelity in cells.

Typ des Eintrags: Artikel
Erschienen: 2014
Autor(en): Borcherds, Wade ; Theillet, François-Xavier ; Katzer, Andrea ; Finzel, Ana ; Mishall, Katie M. ; Powell, Anne T. ; Wu, Hongwei ; Manieri, Wanda ; Dieterich, Christoph ; Selenko, Philipp ; Loewer, Alexander ; Daughdrill, Gary W.
Titel: Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Levels of residual structure in disordered interaction domains determine in vitro binding affinities, but whether they exert similar roles in cells is not known. Here, we show that increasing residual p53 helicity results in stronger Mdm2 binding, altered p53 dynamics, impaired target gene expression and failure to induce cell cycle arrest upon DNA damage. These results establish that residual structure is an important determinant of signaling fidelity in cells.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Nature chemical biology
Band: 10
(Heft-)Nummer: 12
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology
Fachbereich Biologie, Biology > Molekularbiologie der Stressantwort
Hinterlegungsdatum: 02 Sep 2015 08:29
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