Borcherds, Wade ; Theillet, François-Xavier ; Katzer, Andrea ; Finzel, Ana ; Mishall, Katie M. ; Powell, Anne T. ; Wu, Hongwei ; Manieri, Wanda ; Dieterich, Christoph ; Selenko, Philipp ; Loewer, Alexander ; Daughdrill, Gary W. (2014)
Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells.
In: Nature chemical biology, 10 (12)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Levels of residual structure in disordered interaction domains determine in vitro binding affinities, but whether they exert similar roles in cells is not known. Here, we show that increasing residual p53 helicity results in stronger Mdm2 binding, altered p53 dynamics, impaired target gene expression and failure to induce cell cycle arrest upon DNA damage. These results establish that residual structure is an important determinant of signaling fidelity in cells.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2014 |
| Autor(en): | Borcherds, Wade ; Theillet, François-Xavier ; Katzer, Andrea ; Finzel, Ana ; Mishall, Katie M. ; Powell, Anne T. ; Wu, Hongwei ; Manieri, Wanda ; Dieterich, Christoph ; Selenko, Philipp ; Loewer, Alexander ; Daughdrill, Gary W. |
| Art des Eintrags: | Bibliographie |
| Titel: | Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells. |
| Sprache: | Englisch |
| Publikationsjahr: | Dezember 2014 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nature chemical biology |
| Jahrgang/Volume einer Zeitschrift: | 10 |
| (Heft-)Nummer: | 12 |
| Kurzbeschreibung (Abstract): | Levels of residual structure in disordered interaction domains determine in vitro binding affinities, but whether they exert similar roles in cells is not known. Here, we show that increasing residual p53 helicity results in stronger Mdm2 binding, altered p53 dynamics, impaired target gene expression and failure to induce cell cycle arrest upon DNA damage. These results establish that residual structure is an important determinant of signaling fidelity in cells. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Systems Biology of the Stress Response |
| Hinterlegungsdatum: | 02 Sep 2015 08:29 |
| Letzte Änderung: | 02 Sep 2015 08:29 |
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