Sator, Christian ; Lico, Chiara ; Pannucci, Elisa ; Marchetti, Luca ; Baschieri, Selene ; Warzecha, Heribert ; Santi, Luca (2024)
Plant-Produced Viral Nanoparticles as a Functionalized Catalytic Support for Metabolic Engineering.
In: Plants, 2024, 13 (4)
doi: 10.26083/tuprints-00027158
Artikel, Zweitveröffentlichung, Verlagsversion
 | Es ist eine neuere Version dieses Eintrags verfügbar. |
Kurzbeschreibung (Abstract)
Substrate channeling could be very useful for plant metabolic engineering; hence, we propose that functionalized supramolecular self-assembly scaffolds can act as enzymatic hubs able to perform reactions in close contiguity. Virus nanoparticles (VNPs) offer an opportunity in this context, and we present a functionalization strategy to display different enzymes on the outer surface of three different VNPs produced in plants. Tomato bushy stunt virus (TBSV) and Potato virus X (PVX) plant viruses were functionalized by the genetic fusion of the E-coil peptide coding sequence to their respective coat proteins genes, while the enzyme lichenase was tagged with the K-coil peptide. Immobilized E-coil VNPs were able to interact in vitro with the plant-produced functionalized lichenase, and catalysis was demonstrated by employing a lichenase assay. To prove this concept in planta, the Hepatitis B core (HBc) virus-like particles (VLPs) were similarly functionalized by genetic fusion with the E-coil sequence, while acyl-activating enzyme 1, olivetolic acid synthase, and olivetolic acid cyclase enzymes were tagged with the K-coil. The transient co-expression of the K-coil-enzymes together with E-coil-VLPs allowed the establishment of the heterologous cannabinoid precursor biosynthetic pathway. Noteworthy, a significantly higher yield of olivetolic acid glucoside was achieved when the scaffold E-coil-VLPs were employed.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Sator, Christian ; Lico, Chiara ; Pannucci, Elisa ; Marchetti, Luca ; Baschieri, Selene ; Warzecha, Heribert ; Santi, Luca |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Plant-Produced Viral Nanoparticles as a Functionalized Catalytic Support for Metabolic Engineering |
| Sprache: | Englisch |
| Publikationsjahr: | 14 Mai 2024 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 11 Februar 2024 |
| Ort der Erstveröffentlichung: | Basel |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Plants |
| Jahrgang/Volume einer Zeitschrift: | 13 |
| (Heft-)Nummer: | 4 |
| Kollation: | 14 Seiten |
| DOI: | 10.26083/tuprints-00027158 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/27158 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | Substrate channeling could be very useful for plant metabolic engineering; hence, we propose that functionalized supramolecular self-assembly scaffolds can act as enzymatic hubs able to perform reactions in close contiguity. Virus nanoparticles (VNPs) offer an opportunity in this context, and we present a functionalization strategy to display different enzymes on the outer surface of three different VNPs produced in plants. Tomato bushy stunt virus (TBSV) and Potato virus X (PVX) plant viruses were functionalized by the genetic fusion of the E-coil peptide coding sequence to their respective coat proteins genes, while the enzyme lichenase was tagged with the K-coil peptide. Immobilized E-coil VNPs were able to interact in vitro with the plant-produced functionalized lichenase, and catalysis was demonstrated by employing a lichenase assay. To prove this concept in planta, the Hepatitis B core (HBc) virus-like particles (VLPs) were similarly functionalized by genetic fusion with the E-coil sequence, while acyl-activating enzyme 1, olivetolic acid synthase, and olivetolic acid cyclase enzymes were tagged with the K-coil. The transient co-expression of the K-coil-enzymes together with E-coil-VLPs allowed the establishment of the heterologous cannabinoid precursor biosynthetic pathway. Noteworthy, a significantly higher yield of olivetolic acid glucoside was achieved when the scaffold E-coil-VLPs were employed. |
| Freie Schlagworte: | plant metabolic engineering, plant virus nanoparticles, plant-produced virus-like particles, multi-enzymatic assemblies, olivetolic acid, cannabinoids |
| ID-Nummer: | Artikel-ID: 503 |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-271587 |
| Zusätzliche Informationen: | This article belongs to the Special Issue Plant Metabolic Engineering |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 500 Naturwissenschaften und Mathematik > 580 Pflanzen (Botanik) |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering Interdisziplinäre Forschungsprojekte Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology |
| Hinterlegungsdatum: | 14 Mai 2024 13:44 |
| Letzte Änderung: | 15 Mai 2024 05:19 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
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