Sator, Christian ; Lico, Chiara ; Pannucci, Elisa ; Marchetti, Luca ; Baschieri, Selene ; Warzecha, Heribert ; Santi, Luca (2024)
Plant-produced viral nanoparticles as a functionalized catalytic support for metabolic engineering.
In: Plants (Basel, Switzerland), 13 (4)
doi: 10.3390/plants13040503
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Substrate channeling could be very useful for plant metabolic engineering; hence, we propose that functionalized supramolecular self-assembly scaffolds can act as enzymatic hubs able to perform reactions in close contiguity. Virus nanoparticles (VNPs) offer an opportunity in this context, and we present a functionalization strategy to display different enzymes on the outer surface of three different VNPs produced in plants. Tomato bushy stunt virus (TBSV) and Potato virus X (PVX) plant viruses were functionalized by the genetic fusion of the E-coil peptide coding sequence to their respective coat proteins genes, while the enzyme lichenase was tagged with the K-coil peptide. Immobilized E-coil VNPs were able to interact with the plant-produced functionalized lichenase, and catalysis was demonstrated by employing a lichenase assay. To prove this concept , the Hepatitis B core (HBc) virus-like particles (VLPs) were similarly functionalized by genetic fusion with the E-coil sequence, while acyl-activating enzyme 1, olivetolic acid synthase, and olivetolic acid cyclase enzymes were tagged with the K-coil. The transient co-expression of the K-coil-enzymes together with E-coil-VLPs allowed the establishment of the heterologous cannabinoid precursor biosynthetic pathway. Noteworthy, a significantly higher yield of olivetolic acid glucoside was achieved when the scaffold E-coil-VLPs were employed.

Frage zum Eintrag

Redaktionelle Details anzeigen