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Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Patron, Nicola J. ; Orzaez, Diego ; Marillonnet, Sylvestre ; Warzecha, Heribert ; Matthewman, Colette ; Youles, Mark ; Raitskin, Oleg ; Leveau, Aymeric ; Farré, Gemma ; Rogers, Christian ; Smith, Alison ; Hibberd, Julian ; Webb, Alex A. R. ; Locke, James ; Schornack, Sebastian ; Ajioka, Jim ; Baulcombe, David C. ; Zipfel, Cyril ; Kamoun, Sophien ; Jones, Jonathan D. G. ; Kuhn, Hannah ; Robatzek, Silke ; Esse, H. Peter van ; Sanders, Dale ; Oldroyd, Giles ; Martin, Cathie ; Field, Rob ; O'Connor, Sarah ; Fox, Samantha ; Wulff, Brande ; Miller, Ben ; Breakspear, Andy ; Radhakrishnan, Guru ; Delaux, Pierre-Marc ; Loqué, Dominique ; Granell, Antonio ; Tissier, Alain ; Shih, Patrick ; Brutnell, Thomas P. ; Quick, W. Paul ; Rischer, Heiko ; Fraser, Paul D. ; Aharoni, Asaph ; Raines, Christine ; South, Paul F. ; Ané, Jean-Michel ; Hamberger, Björn R. ; Langdale, Jane ; Stougaard, Jens ; Bouwmeester, Harro ; Udvardi, Michael ; Murray, James A. H. ; Ntoukakis, Vardis ; Schäfer, Patrick ; Denby, Katherine ; Edwards, Keith J. ; Osbourn, Anne ; Haseloff, Jim (2015)
Standards for plant synthetic biology: a common syntax for exchange of DNA parts.
In: The New phytologist, 208 (1)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Typ des Eintrags: Artikel
Erschienen: 2015
Autor(en): Patron, Nicola J. ; Orzaez, Diego ; Marillonnet, Sylvestre ; Warzecha, Heribert ; Matthewman, Colette ; Youles, Mark ; Raitskin, Oleg ; Leveau, Aymeric ; Farré, Gemma ; Rogers, Christian ; Smith, Alison ; Hibberd, Julian ; Webb, Alex A. R. ; Locke, James ; Schornack, Sebastian ; Ajioka, Jim ; Baulcombe, David C. ; Zipfel, Cyril ; Kamoun, Sophien ; Jones, Jonathan D. G. ; Kuhn, Hannah ; Robatzek, Silke ; Esse, H. Peter van ; Sanders, Dale ; Oldroyd, Giles ; Martin, Cathie ; Field, Rob ; O'Connor, Sarah ; Fox, Samantha ; Wulff, Brande ; Miller, Ben ; Breakspear, Andy ; Radhakrishnan, Guru ; Delaux, Pierre-Marc ; Loqué, Dominique ; Granell, Antonio ; Tissier, Alain ; Shih, Patrick ; Brutnell, Thomas P. ; Quick, W. Paul ; Rischer, Heiko ; Fraser, Paul D. ; Aharoni, Asaph ; Raines, Christine ; South, Paul F. ; Ané, Jean-Michel ; Hamberger, Björn R. ; Langdale, Jane ; Stougaard, Jens ; Bouwmeester, Harro ; Udvardi, Michael ; Murray, James A. H. ; Ntoukakis, Vardis ; Schäfer, Patrick ; Denby, Katherine ; Edwards, Keith J. ; Osbourn, Anne ; Haseloff, Jim
Art des Eintrags: Bibliographie
Titel: Standards for plant synthetic biology: a common syntax for exchange of DNA parts
Sprache: Englisch
Publikationsjahr: 2015
Titel der Zeitschrift, Zeitung oder Schriftenreihe: The New phytologist
Jahrgang/Volume einer Zeitschrift: 208
(Heft-)Nummer: 1
Kurzbeschreibung (Abstract):

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering
Hinterlegungsdatum: 20 Jul 2015 12:05
Letzte Änderung: 23 Jul 2021 12:33
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