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Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Patron, Nicola J. ; Orzaez, Diego ; Marillonnet, Sylvestre ; Warzecha, Heribert ; Matthewman, Colette ; Youles, Mark ; Raitskin, Oleg ; Leveau, Aymeric ; Farré, Gemma ; Rogers, Christian ; Smith, Alison ; Hibberd, Julian ; Webb, Alex A. R. ; Locke, James ; Schornack, Sebastian ; Ajioka, Jim ; Baulcombe, David C. ; Zipfel, Cyril ; Kamoun, Sophien ; Jones, Jonathan D. G. ; Kuhn, Hannah ; Robatzek, Silke ; Esse, H. Peter van ; Sanders, Dale ; Oldroyd, Giles ; Martin, Cathie ; Field, Rob ; O'Connor, Sarah ; Fox, Samantha ; Wulff, Brande ; Miller, Ben ; Breakspear, Andy ; Radhakrishnan, Guru ; Delaux, Pierre-Marc ; Loqué, Dominique ; Granell, Antonio ; Tissier, Alain ; Shih, Patrick ; Brutnell, Thomas P. ; Quick, W. Paul ; Rischer, Heiko ; Fraser, Paul D. ; Aharoni, Asaph ; Raines, Christine ; South, Paul F. ; Ané, Jean-Michel ; Hamberger, Björn R. ; Langdale, Jane ; Stougaard, Jens ; Bouwmeester, Harro ; Udvardi, Michael ; Murray, James A. H. ; Ntoukakis, Vardis ; Schäfer, Patrick ; Denby, Katherine ; Edwards, Keith J. ; Osbourn, Anne ; Haseloff, Jim (2015):
Standards for plant synthetic biology: a common syntax for exchange of DNA parts.
In: The New phytologist, 208 (1), pp. 13-19. ISSN 1469-8137,
[Article]

Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Item Type: Article
Erschienen: 2015
Creators: Patron, Nicola J. ; Orzaez, Diego ; Marillonnet, Sylvestre ; Warzecha, Heribert ; Matthewman, Colette ; Youles, Mark ; Raitskin, Oleg ; Leveau, Aymeric ; Farré, Gemma ; Rogers, Christian ; Smith, Alison ; Hibberd, Julian ; Webb, Alex A. R. ; Locke, James ; Schornack, Sebastian ; Ajioka, Jim ; Baulcombe, David C. ; Zipfel, Cyril ; Kamoun, Sophien ; Jones, Jonathan D. G. ; Kuhn, Hannah ; Robatzek, Silke ; Esse, H. Peter van ; Sanders, Dale ; Oldroyd, Giles ; Martin, Cathie ; Field, Rob ; O'Connor, Sarah ; Fox, Samantha ; Wulff, Brande ; Miller, Ben ; Breakspear, Andy ; Radhakrishnan, Guru ; Delaux, Pierre-Marc ; Loqué, Dominique ; Granell, Antonio ; Tissier, Alain ; Shih, Patrick ; Brutnell, Thomas P. ; Quick, W. Paul ; Rischer, Heiko ; Fraser, Paul D. ; Aharoni, Asaph ; Raines, Christine ; South, Paul F. ; Ané, Jean-Michel ; Hamberger, Björn R. ; Langdale, Jane ; Stougaard, Jens ; Bouwmeester, Harro ; Udvardi, Michael ; Murray, James A. H. ; Ntoukakis, Vardis ; Schäfer, Patrick ; Denby, Katherine ; Edwards, Keith J. ; Osbourn, Anne ; Haseloff, Jim
Title: Standards for plant synthetic biology: a common syntax for exchange of DNA parts
Language: English
Abstract:

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Journal or Publication Title: The New phytologist
Journal volume: 208
Number: 1
Divisions: 10 Department of Biology
10 Department of Biology > Plant Biotechnology and Metabolic Engineering
Date Deposited: 20 Jul 2015 12:05
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