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The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study.

Meier, N. and Göringer, H. Ulrich and Kleuvers, B. and Scheibe, U. and Eberle, J. and Szymkowiak, C. and Zacharias, M. and Wagner, R. (1986):
The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study.
In: FEBS letters, pp. 89-95, 204, (1), ISSN 0014-5793,
[Article]

Abstract

Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of defined nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.

Item Type: Article
Erschienen: 1986
Creators: Meier, N. and Göringer, H. Ulrich and Kleuvers, B. and Scheibe, U. and Eberle, J. and Szymkowiak, C. and Zacharias, M. and Wagner, R.
Title: The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study.
Language: English
Abstract:

Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of defined nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.

Journal or Publication Title: FEBS letters
Volume: 204
Number: 1
Divisions: 10 Department of Biology > Postranscriptional Gene Regulation and RNA Therapeutics
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10 Department of Biology
Date Deposited: 07 Nov 2011 09:07
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