Meier, N. ; Göringer, H. Ulrich ; Kleuvers, B. ; Scheibe, U. ; Eberle, J. ; Szymkowiak, C. ; Zacharias, M. ; Wagner, R. (1986)
The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study.
In: FEBS letters, 204 (1)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of defined nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1986 |
| Autor(en): | Meier, N. ; Göringer, H. Ulrich ; Kleuvers, B. ; Scheibe, U. ; Eberle, J. ; Szymkowiak, C. ; Zacharias, M. ; Wagner, R. |
| Art des Eintrags: | Bibliographie |
| Titel: | The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study. |
| Sprache: | Englisch |
| Publikationsjahr: | 1986 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 204 |
| (Heft-)Nummer: | 1 |
| Kurzbeschreibung (Abstract): | Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of defined nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Genregulation und RNA-Therapeutika ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 07 Nov 2011 09:07 |
| Letzte Änderung: | 05 Mär 2013 09:55 |
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