Prescott, C. D. ; Kleuvers, B. ; Göringer, H. Ulrich (2011)
A rRNA-mRNA base pairing model for UGA-dependent termination.
In: Biochimie, 73 (7-8)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UGA-dependent translational termination. With the exception of the C726 to G base change, all were constructed in helix 34. Characterization of these mutations is reviewed here and from these data and mRNA-rRNA base pairing model for the termination event is presented. The interaction functions via antiparallel base pairing between either 1 of the 2 UCA motifs in helix 34 and the complementary UGA stop codon on the message, thus forming a quasicontinuous A-type helical structure that is further stabilized by stacking enthalpy. Finally, rRNA motifs potentially required for UAA and UAG-dependent translational termination are discussed.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2011 |
| Autor(en): | Prescott, C. D. ; Kleuvers, B. ; Göringer, H. Ulrich |
| Art des Eintrags: | Bibliographie |
| Titel: | A rRNA-mRNA base pairing model for UGA-dependent termination. |
| Sprache: | Englisch |
| Publikationsjahr: | 3 November 2011 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biochimie |
| Jahrgang/Volume einer Zeitschrift: | 73 |
| (Heft-)Nummer: | 7-8 |
| Kurzbeschreibung (Abstract): | A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UGA-dependent translational termination. With the exception of the C726 to G base change, all were constructed in helix 34. Characterization of these mutations is reviewed here and from these data and mRNA-rRNA base pairing model for the termination event is presented. The interaction functions via antiparallel base pairing between either 1 of the 2 UCA motifs in helix 34 and the complementary UGA stop codon on the message, thus forming a quasicontinuous A-type helical structure that is further stabilized by stacking enthalpy. Finally, rRNA motifs potentially required for UAA and UAG-dependent translational termination are discussed. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Genregulation und RNA-Therapeutika ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 03 Nov 2011 13:54 |
| Letzte Änderung: | 05 Mär 2013 09:55 |
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