O'Connor, M. ; Göringer, H. Ulrich ; Dahlberg, A. E. (1992)
A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.
In: Nucleic acids research, 20 (16)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1992 |
| Autor(en): | O'Connor, M. ; Göringer, H. Ulrich ; Dahlberg, A. E. |
| Art des Eintrags: | Bibliographie |
| Titel: | A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA. |
| Sprache: | Englisch |
| Publikationsjahr: | 1992 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nucleic acids research |
| Jahrgang/Volume einer Zeitschrift: | 20 |
| (Heft-)Nummer: | 16 |
| Kurzbeschreibung (Abstract): | A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Genregulation und RNA-Therapeutika ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 03 Nov 2011 13:50 |
| Letzte Änderung: | 05 Mär 2013 09:55 |
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