TU Darmstadt / ULB / TUbiblio

A three-dimensional working model for a guide RNA from Trypanosoma brucei.

Hermann, T. ; Schmid, B. ; Heumann, H. ; Göringer, H. Ulrich (1997)
A three-dimensional working model for a guide RNA from Trypanosoma brucei.
In: Nucleic acids research, 25 (12)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

RNA editing in protozoan parasites is a mitochondrial RNA processing reaction in which exclusively uridylate residues are inserted into, and less frequently deleted from, pre-mRNAs. Molecules central to the process are so-called guide RNAs (gRNAs) which function as templates in the reaction. For a detailed molecular understanding of the mechanism of the editing process knowledge of structural features of gRNAs will be essential. Here we report on a computer-assisted molecular modelling approach to construct the first three-dimensional gRNA model for gND7-506, a ND7-specific gRNA from Trypanosoma brucei. The modelling process relied on chemical modification and enzymatic probing data and was validated by in vitro mutagenesis experiments. The model predicts a reasonably compact structure, where two stem/loop secondary structure elements are brought into close proximity by a triple A tertiary interaction, forming a core element within the centre of the molecule. The model further suggests that the surface of the gRNA is primarily made up of the sugar-phoshate backbone. On the basis of the model, footprinting experiments of gND7-506 in a complex with the gRNA binding protein gBP21 could successfully be interpreted and provide a first picture for the assembly of gRNAs within a ribonucleoprotein complex.

Typ des Eintrags: Artikel
Erschienen: 1997
Autor(en): Hermann, T. ; Schmid, B. ; Heumann, H. ; Göringer, H. Ulrich
Art des Eintrags: Bibliographie
Titel: A three-dimensional working model for a guide RNA from Trypanosoma brucei.
Sprache: Englisch
Publikationsjahr: 1997
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Nucleic acids research
Jahrgang/Volume einer Zeitschrift: 25
(Heft-)Nummer: 12
Kurzbeschreibung (Abstract):

RNA editing in protozoan parasites is a mitochondrial RNA processing reaction in which exclusively uridylate residues are inserted into, and less frequently deleted from, pre-mRNAs. Molecules central to the process are so-called guide RNAs (gRNAs) which function as templates in the reaction. For a detailed molecular understanding of the mechanism of the editing process knowledge of structural features of gRNAs will be essential. Here we report on a computer-assisted molecular modelling approach to construct the first three-dimensional gRNA model for gND7-506, a ND7-specific gRNA from Trypanosoma brucei. The modelling process relied on chemical modification and enzymatic probing data and was validated by in vitro mutagenesis experiments. The model predicts a reasonably compact structure, where two stem/loop secondary structure elements are brought into close proximity by a triple A tertiary interaction, forming a core element within the centre of the molecule. The model further suggests that the surface of the gRNA is primarily made up of the sugar-phoshate backbone. On the basis of the model, footprinting experiments of gND7-506 in a complex with the gRNA binding protein gBP21 could successfully be interpreted and provide a first picture for the assembly of gRNAs within a ribonucleoprotein complex.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Genregulation und RNA-Therapeutika
?? fb10_mikrobiologie ??
10 Fachbereich Biologie
Hinterlegungsdatum: 03 Nov 2011 13:25
Letzte Änderung: 05 Mär 2013 09:55
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Frage zum Eintrag Frage zum Eintrag

Optionen (nur für Redakteure)
Redaktionelle Details anzeigen Redaktionelle Details anzeigen