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Combinatorial selection of high affinity RNA ligands to live African trypanosomes.

Homann, M. ; Göringer, H. Ulrich (1999)
Combinatorial selection of high affinity RNA ligands to live African trypanosomes.
In: Nucleic acids research, 27 (9)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

African trypanosomiasis is a parasitic disease caused by a specific class of protozoan organisms. The best-studied representative of that group is Trypanosoma brucei which is transmitted by tsetse flies and multiplies in the blood of many mammals. Trypanosomes evade the immune system by altering their surface structure which is dominated by a layer of a variant surface glycoprotein (VSG). Although invariant surface proteins exist, they are inaccessible to the humoral immune response. Using a combinatorial selection method in conjunction with live trypanosomes as the binding target, we show that short RNA ligands (aptamers) for constant surface components can be isolated. We describe the selection of three classes of RNA aptamers that crosslink to a single 42 kDa protein located within the flagellar pocket of the parasite. The RNAs associate rapidly and with high affinity. They do not discriminate between two different trypanosome VSG variant strains and, furthermore, are able to bind to other trypanosome strains not used in the selection protocol. Thus, the aptamers have the potential to function as markers on the surface of the extracellular parasite and as such they might be modified to function as novel drugs against African trypanosomiasis.

Typ des Eintrags: Artikel
Erschienen: 1999
Autor(en): Homann, M. ; Göringer, H. Ulrich
Art des Eintrags: Bibliographie
Titel: Combinatorial selection of high affinity RNA ligands to live African trypanosomes.
Sprache: Englisch
Publikationsjahr: 1999
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Nucleic acids research
Jahrgang/Volume einer Zeitschrift: 27
(Heft-)Nummer: 9
Kurzbeschreibung (Abstract):

African trypanosomiasis is a parasitic disease caused by a specific class of protozoan organisms. The best-studied representative of that group is Trypanosoma brucei which is transmitted by tsetse flies and multiplies in the blood of many mammals. Trypanosomes evade the immune system by altering their surface structure which is dominated by a layer of a variant surface glycoprotein (VSG). Although invariant surface proteins exist, they are inaccessible to the humoral immune response. Using a combinatorial selection method in conjunction with live trypanosomes as the binding target, we show that short RNA ligands (aptamers) for constant surface components can be isolated. We describe the selection of three classes of RNA aptamers that crosslink to a single 42 kDa protein located within the flagellar pocket of the parasite. The RNAs associate rapidly and with high affinity. They do not discriminate between two different trypanosome VSG variant strains and, furthermore, are able to bind to other trypanosome strains not used in the selection protocol. Thus, the aptamers have the potential to function as markers on the surface of the extracellular parasite and as such they might be modified to function as novel drugs against African trypanosomiasis.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Genregulation und RNA-Therapeutika
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10 Fachbereich Biologie
Hinterlegungsdatum: 03 Nov 2011 13:16
Letzte Änderung: 05 Mär 2013 09:55
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