Dogru, E. ; Warzecha, Heribert ; Seibel, F. ; Haebel, S. ; Lottspeich, F. ; Stöckigt, J. (2000)
The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family.
In: European journal of biochemistry / FEBS, 267 (5)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The biosynthesis of the anti-arrhythmic alkaloid ajmaline is catalysed by more than 10 specific enzymes. In this multistep process polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. PNAE was purified from cell suspension cultures of Rauvolfia serpentina. The N-terminal sequence and endoproteinase LysC fragments of the purified protein were used for primer design and for the amplification of specific PCR products leading to the isolation of PNAE-encoding cDNA from a R. serpentina library. The PNAE cDNA was fused with a C-terminal His-tag, expressed in Escherichia coli and purified to homogeneity using Ni-affinity chromatography. The pure enzyme shows extraordinary substrate specificity, completely different to other esterases. Sequence alignments indicate that PNAE is a new member of the alpha/beta hydrolase super family.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2000 |
| Autor(en): | Dogru, E. ; Warzecha, Heribert ; Seibel, F. ; Haebel, S. ; Lottspeich, F. ; Stöckigt, J. |
| Art des Eintrags: | Bibliographie |
| Titel: | The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family. |
| Sprache: | Englisch |
| Publikationsjahr: | 2000 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | European journal of biochemistry / FEBS |
| Jahrgang/Volume einer Zeitschrift: | 267 |
| (Heft-)Nummer: | 5 |
| Kurzbeschreibung (Abstract): | The biosynthesis of the anti-arrhythmic alkaloid ajmaline is catalysed by more than 10 specific enzymes. In this multistep process polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. PNAE was purified from cell suspension cultures of Rauvolfia serpentina. The N-terminal sequence and endoproteinase LysC fragments of the purified protein were used for primer design and for the amplification of specific PCR products leading to the isolation of PNAE-encoding cDNA from a R. serpentina library. The PNAE cDNA was fused with a C-terminal His-tag, expressed in Escherichia coli and purified to homogeneity using Ni-affinity chromatography. The pure enzyme shows extraordinary substrate specificity, completely different to other esterases. Sequence alignments indicate that PNAE is a new member of the alpha/beta hydrolase super family. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 17 Mär 2011 12:26 |
| Letzte Änderung: | 05 Mär 2013 09:46 |
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