Dueckershoff, K. ; Unger, M. ; Frank, A. ; Gillam, E. M. J. ; Guengerich, F. P. ; Warzecha, Heribert (2005)
Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.
In: FEBS letters, 579 (11)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
In this study, the human cytochrome P450 (CYP) 2A6 was used in order to modify the alkaloid production of tobacco plants. The cDNA for human CYP2A6 was placed under the control of the constitutive 35S promoter and transferred into Nicotiana tabacum via Agrobacterium-mediated transformation. Transgenic plants showed formation of the recombinant CYP2A6 enzyme but no obvious phenotypic changes. Unlike wild-type tobacco, the transgenic plants accumulated cotinine, a metabolite which is usually formed from nicotine in humans. This result substantiates that metabolic engineering of the plant secondary metabolism via mammalian P450 enzymes is possible in vivo.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2005 |
| Autor(en): | Dueckershoff, K. ; Unger, M. ; Frank, A. ; Gillam, E. M. J. ; Guengerich, F. P. ; Warzecha, Heribert |
| Art des Eintrags: | Bibliographie |
| Titel: | Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA. |
| Sprache: | Englisch |
| Publikationsjahr: | 2005 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 579 |
| (Heft-)Nummer: | 11 |
| Kurzbeschreibung (Abstract): | In this study, the human cytochrome P450 (CYP) 2A6 was used in order to modify the alkaloid production of tobacco plants. The cDNA for human CYP2A6 was placed under the control of the constitutive 35S promoter and transferred into Nicotiana tabacum via Agrobacterium-mediated transformation. Transgenic plants showed formation of the recombinant CYP2A6 enzyme but no obvious phenotypic changes. Unlike wild-type tobacco, the transgenic plants accumulated cotinine, a metabolite which is usually formed from nicotine in humans. This result substantiates that metabolic engineering of the plant secondary metabolism via mammalian P450 enzymes is possible in vivo. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 17 Mär 2011 11:03 |
| Letzte Änderung: | 05 Mär 2013 09:46 |
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