Stanbekova, Gulshan ; Beisenov, Daniyar ; Nizkorodova, Anna ; Iskakov, Bulat ; Warzecha, Heribert (2021)
Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts.
In: Biotechnology Letters, 43 (7)
doi: 10.1007/s10529-021-03117-x
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Objective: A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox.
Results: Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g⁻¹ (FW). The serum of a sheep infected with the virus recognised the chloroplast-produced protein indicating that the protein retains its antigenic properties.
Conclusions: These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Stanbekova, Gulshan ; Beisenov, Daniyar ; Nizkorodova, Anna ; Iskakov, Bulat ; Warzecha, Heribert |
| Art des Eintrags: | Bibliographie |
| Titel: | Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts |
| Sprache: | Englisch |
| Publikationsjahr: | Juli 2021 |
| Ort: | Dordrecht |
| Verlag: | Springer Science |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biotechnology Letters |
| Jahrgang/Volume einer Zeitschrift: | 43 |
| (Heft-)Nummer: | 7 |
| DOI: | 10.1007/s10529-021-03117-x |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Objective: A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox. Results: Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g⁻¹ (FW). The serum of a sheep infected with the virus recognised the chloroplast-produced protein indicating that the protein retains its antigenic properties. Conclusions: These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox. |
| Freie Schlagworte: | Sheep pox virus, SPPV117 protein, Transplastomic plants |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 500 Naturwissenschaften und Mathematik > 580 Pflanzen (Botanik) |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering |
| Hinterlegungsdatum: | 19 Dez 2024 08:48 |
| Letzte Änderung: | 19 Dez 2024 13:19 |
| PPN: | 52485520X |
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| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts. (deposited 17 Dez 2024 12:25)
- Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts. (deposited 19 Dez 2024 08:48) [Gegenwärtig angezeigt]
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