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Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts

Stanbekova, Gulshan ; Beisenov, Daniyar ; Nizkorodova, Anna ; Iskakov, Bulat ; Warzecha, Heribert (2024)
Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts.
In: Biotechnology Letters, 2021, 43 (7)
doi: 10.26083/tuprints-00023491
Artikel, Zweitveröffentlichung, Verlagsversion

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Kurzbeschreibung (Abstract)

Objective: A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox.

Results: Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g⁻¹ (FW). The serum of a sheep infected with the virus recognised the chloroplast-produced protein indicating that the protein retains its antigenic properties.

Conclusions: These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox.

Typ des Eintrags: Artikel
Erschienen: 2024
Autor(en): Stanbekova, Gulshan ; Beisenov, Daniyar ; Nizkorodova, Anna ; Iskakov, Bulat ; Warzecha, Heribert
Art des Eintrags: Zweitveröffentlichung
Titel: Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts
Sprache: Englisch
Publikationsjahr: 17 Dezember 2024
Ort: Darmstadt
Publikationsdatum der Erstveröffentlichung: Juli 2021
Ort der Erstveröffentlichung: Dordrecht
Verlag: Springer Science
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Biotechnology Letters
Jahrgang/Volume einer Zeitschrift: 43
(Heft-)Nummer: 7
DOI: 10.26083/tuprints-00023491
URL / URN: https://tuprints.ulb.tu-darmstadt.de/23491
Zugehörige Links:
Herkunft: Zweitveröffentlichung DeepGreen
Kurzbeschreibung (Abstract):

Objective: A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox.

Results: Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g⁻¹ (FW). The serum of a sheep infected with the virus recognised the chloroplast-produced protein indicating that the protein retains its antigenic properties.

Conclusions: These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox.

Freie Schlagworte: Sheep pox virus, SPPV117 protein, Transplastomic plants
Status: Verlagsversion
URN: urn:nbn:de:tuda-tuprints-234919
Sachgruppe der Dewey Dezimalklassifikatin (DDC): 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
500 Naturwissenschaften und Mathematik > 580 Pflanzen (Botanik)
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering
Hinterlegungsdatum: 17 Dez 2024 12:25
Letzte Änderung: 19 Dez 2024 08:45
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