Schröter, Christian ; Becker, Jan ; Krah, Simon ; Zielonka, Stefan ; Doerner, Achim ; Rhiel, Laura ; Günther, Ralf ; Toleikis, Lars ; Kolmar, Harald ; Hock, Björn ; Becker, Stefan (2018)
Selection of antibodies with tailored properties by application of high-throughput multiparameter fluorescence-activated cell sorting of yeast-displayed immune libraries.
In: Molecular Biotechnology, 60
doi: 10.1007/s12033-018-0109-0
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
In this study, we present a multiparameter screening procedure for the identification of target-specific antibodies with prescribed properties. Based on B cell receptor gene repertoires from transgenic rats, yeast surface display libraries were generated, and high-affinity human antibodies were readily isolated. We demonstrate that specific desirable features, i.e., species’ cross-reactivity and a broad epitope coverage can be integrated into the screening procedure using high-throughput fluorescence-activated cell sorting. We show that the applied screening stringencies translate directly into binding properties of isolated human antibody variants.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2018 |
| Autor(en): | Schröter, Christian ; Becker, Jan ; Krah, Simon ; Zielonka, Stefan ; Doerner, Achim ; Rhiel, Laura ; Günther, Ralf ; Toleikis, Lars ; Kolmar, Harald ; Hock, Björn ; Becker, Stefan |
| Art des Eintrags: | Bibliographie |
| Titel: | Selection of antibodies with tailored properties by application of high-throughput multiparameter fluorescence-activated cell sorting of yeast-displayed immune libraries |
| Sprache: | Englisch |
| Publikationsjahr: | 2018 |
| Ort: | New York, NY |
| Verlag: | Springer |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Molecular Biotechnology |
| Jahrgang/Volume einer Zeitschrift: | 60 |
| DOI: | 10.1007/s12033-018-0109-0 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | In this study, we present a multiparameter screening procedure for the identification of target-specific antibodies with prescribed properties. Based on B cell receptor gene repertoires from transgenic rats, yeast surface display libraries were generated, and high-affinity human antibodies were readily isolated. We demonstrate that specific desirable features, i.e., species’ cross-reactivity and a broad epitope coverage can be integrated into the screening procedure using high-throughput fluorescence-activated cell sorting. We show that the applied screening stringencies translate directly into binding properties of isolated human antibody variants. |
| Freie Schlagworte: | Yeast surface display, Fab display, Affinity, Specificity, Fluorescence-activated cell sorting, Combinatorial immune libraries |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 07 Fachbereich Chemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut > Fachgebiet Biochemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut |
| Hinterlegungsdatum: | 20 Aug 2024 06:19 |
| Letzte Änderung: | 20 Aug 2024 06:19 |
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| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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Selection of Antibodies with Tailored Properties by Application of High-Throughput Multiparameter Fluorescence-Activated Cell Sorting of Yeast-Displayed Immune Libraries. (deposited 19 Aug 2024 09:49)
- Selection of antibodies with tailored properties by application of high-throughput multiparameter fluorescence-activated cell sorting of yeast-displayed immune libraries. (deposited 20 Aug 2024 06:19) [Gegenwärtig angezeigt]
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