TU Darmstadt / ULB / TUbiblio

Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways

Geissler, Marcus ; Neubauer, Christoph ; Sheludko, Yuriy V. ; Brückner, Adrian ; Warzecha, Heribert (2024)
Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways.
In: Biotechnology letters, 46 (5)
doi: 10.1007/s10529-024-03489-w
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

OBJECTIVE

Evaluation of Nepeta cataria as a host with specific endogenous metabolite background for transient expression and metabolic engineering of secondary biosynthetic sequences.

RESULTS

The reporter gene gfp::licBM3 as well as three biosynthetic genes leading to the formation of the cannabinoid precursor olivetolic acid were adopted to the modular cloning standard GoldenBraid, transiently expressed in two chemotypes of N. cataria and compared to Nicotiana benthamiana. To estimate the expression efficiency in both hosts, quantification of the reporter activity was carried out with a sensitive and specific lichenase assay. While N. benthamiana exhibited lichenase activity of 676 ± 94 μmol g s, N. cataria cultivar '1000', and the cultivar 'Citriodora' showed an activity of 37 ± 8 μmol g s and 18 ± 4 μmol g s, respectively. Further, combinatorial expression of genes involved in cannabinoid biosynthetic pathway acyl-activating enzyme 1 (aae1), olivetol synthase (ols) and olivetolic acid cyclase (oac) in N. cataria cv. resulted presumably in the in vivo production of olivetolic acid glycosides.

CONCLUSION

Nepeta cataria is amenable to Agrobacterium-mediated transient expression and could serve as a novel chassis for the engineering of secondary metabolic pathways and transient evaluation of heterologous genes.

Typ des Eintrags: Artikel
Erschienen: 2024
Autor(en): Geissler, Marcus ; Neubauer, Christoph ; Sheludko, Yuriy V. ; Brückner, Adrian ; Warzecha, Heribert
Art des Eintrags: Bibliographie
Titel: Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways
Sprache: Englisch
Publikationsjahr: 8 Mai 2024
Verlag: Springer
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Biotechnology letters
Jahrgang/Volume einer Zeitschrift: 46
(Heft-)Nummer: 5
DOI: 10.1007/s10529-024-03489-w
Kurzbeschreibung (Abstract):

OBJECTIVE

Evaluation of Nepeta cataria as a host with specific endogenous metabolite background for transient expression and metabolic engineering of secondary biosynthetic sequences.

RESULTS

The reporter gene gfp::licBM3 as well as three biosynthetic genes leading to the formation of the cannabinoid precursor olivetolic acid were adopted to the modular cloning standard GoldenBraid, transiently expressed in two chemotypes of N. cataria and compared to Nicotiana benthamiana. To estimate the expression efficiency in both hosts, quantification of the reporter activity was carried out with a sensitive and specific lichenase assay. While N. benthamiana exhibited lichenase activity of 676 ± 94 μmol g s, N. cataria cultivar '1000', and the cultivar 'Citriodora' showed an activity of 37 ± 8 μmol g s and 18 ± 4 μmol g s, respectively. Further, combinatorial expression of genes involved in cannabinoid biosynthetic pathway acyl-activating enzyme 1 (aae1), olivetol synthase (ols) and olivetolic acid cyclase (oac) in N. cataria cv. resulted presumably in the in vivo production of olivetolic acid glycosides.

CONCLUSION

Nepeta cataria is amenable to Agrobacterium-mediated transient expression and could serve as a novel chassis for the engineering of secondary metabolic pathways and transient evaluation of heterologous genes.

ID-Nummer: pmid:38717662
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering
Hinterlegungsdatum: 13 Mai 2024 11:55
Letzte Änderung: 07 Okt 2024 07:05
PPN: 518209814
Export:
Suche nach Titel in: TUfind oder in Google
Frage zum Eintrag Frage zum Eintrag

Optionen (nur für Redakteure)
Redaktionelle Details anzeigen Redaktionelle Details anzeigen