Juteršek, Mojca ; Gerasymenko, Iryna M. ; Petek, Marko ; Haumann, Elisabeth ; Vacas, Sandra ; Kallam, Kalyani ; Gianoglio, Silvia ; Navarro-Llopis, Vicente ; Heethoff, Michael ; Fuertes, Ismael Navarro ; Patron, Nicola ; Orzáez, Diego ; Gruden, Kristina ; Warzecha, Heribert ; Baebler, Špela (2024)
Transcriptome-informed identification and characterization of Planococcus citri cis- and trans-isoprenyl diphosphate synthase genes.
In: iScience, 27 (4)
doi: 10.1016/j.isci.2024.109441
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Insect physiology and reproduction depend on several terpenoid compounds, whose biosynthesis is mainly unknown. One enigmatic group of insect monoterpenoids are mealybug sex pheromones, presumably resulting from the irregular coupling activity of unidentified isoprenyl diphosphate synthases (IDSs). Here, we performed a comprehensive search for IDS coding sequences of the pest mealybug . We queried the available genomic and newly generated short- and long-read transcriptomic data and identified 18 putative IDS genes, whose phylogenetic analysis indicates several gene family expansion events. testing confirmed regular short-chain coupling activity with five gene products. With the candidate with highest IDS activity, we also detected low amounts of irregular coupling products, and determined amino acid residues important for chain-length preference and irregular coupling activity. This work therefore provides an important foundation for deciphering terpenoid biosynthesis in mealybugs, including the sex pheromone biosynthesis in .
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Juteršek, Mojca ; Gerasymenko, Iryna M. ; Petek, Marko ; Haumann, Elisabeth ; Vacas, Sandra ; Kallam, Kalyani ; Gianoglio, Silvia ; Navarro-Llopis, Vicente ; Heethoff, Michael ; Fuertes, Ismael Navarro ; Patron, Nicola ; Orzáez, Diego ; Gruden, Kristina ; Warzecha, Heribert ; Baebler, Špela |
| Art des Eintrags: | Bibliographie |
| Titel: | Transcriptome-informed identification and characterization of Planococcus citri cis- and trans-isoprenyl diphosphate synthase genes |
| Sprache: | Englisch |
| Publikationsjahr: | 19 April 2024 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | iScience |
| Jahrgang/Volume einer Zeitschrift: | 27 |
| (Heft-)Nummer: | 4 |
| DOI: | 10.1016/j.isci.2024.109441 |
| Kurzbeschreibung (Abstract): | Insect physiology and reproduction depend on several terpenoid compounds, whose biosynthesis is mainly unknown. One enigmatic group of insect monoterpenoids are mealybug sex pheromones, presumably resulting from the irregular coupling activity of unidentified isoprenyl diphosphate synthases (IDSs). Here, we performed a comprehensive search for IDS coding sequences of the pest mealybug . We queried the available genomic and newly generated short- and long-read transcriptomic data and identified 18 putative IDS genes, whose phylogenetic analysis indicates several gene family expansion events. testing confirmed regular short-chain coupling activity with five gene products. With the candidate with highest IDS activity, we also detected low amounts of irregular coupling products, and determined amino acid residues important for chain-length preference and irregular coupling activity. This work therefore provides an important foundation for deciphering terpenoid biosynthesis in mealybugs, including the sex pheromone biosynthesis in . |
| ID-Nummer: | pmid:38523795 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering |
| Hinterlegungsdatum: | 26 Mär 2024 06:50 |
| Letzte Änderung: | 26 Mär 2024 07:31 |
| PPN: | 516554468 |
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| Suche nach Titel in: | TUfind oder in Google |
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