Schmidt, Sönke ; Schubler, Martin ; Hessinger, Carolin ; Schuster, Christian ; Bertulat, Bianca ; Kithil, Marina ; Cardoso, M. Cristina ; Jakoby, Rolf (2019)
Microwave Induced Electroporation of Adherent Mammalian Cells at 18 GHz.
In: IEEE Access, 2019, 7
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
This paper discusses microwave-induced electroporation as a promising alternative to conventional transfection methods. Adherent C2C12 mouse cells are successfully transfected with a 5TAMRA redlabeled peptide by using a recently developed planar microwave electroporation tool. It allows to monitor the uptake kinetics with live-cell confocal microscopy and is suitable to culture, manipulate, and observe the adherent cells over several days. Viability tests with the Calcein blue AM proof the vitality of the treated cells after 72 h. The question of whether the observed effects are temperature or field induced is tackled. For this reason, comprehensive coupled full-wave electromagnetic-thermal simulations are aligned with temperature measurements. The temperature at the position of the cells does not exceed 34 °C for an input power of 24 dBm. The corresponding electric field strength is evaluated at the position of the cells. A value of 150 V/cm is not exceeded, which is at least a factor of 10 below the field strength of the conventional electroporation. Consequently, almost no cell mortality does occur during the treatment. Comparative thermal tests without a microwave field but with a successively increased temperature up to 42 °C show no uptake. In contrast, the successful uptake follows the pattern of the microwave field although the temperature distribution is homogeneous. We rate this as evidence that the uptake is induced by the high-frequency electromagnetic field rather than the temperature.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Schmidt, Sönke ; Schubler, Martin ; Hessinger, Carolin ; Schuster, Christian ; Bertulat, Bianca ; Kithil, Marina ; Cardoso, M. Cristina ; Jakoby, Rolf |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Microwave Induced Electroporation of Adherent Mammalian Cells at 18 GHz |
| Sprache: | Englisch |
| Publikationsjahr: | 2019 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2019 |
| Verlag: | IEEE |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | IEEE Access |
| Jahrgang/Volume einer Zeitschrift: | 7 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/9296 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung aus gefördertem Golden Open Access |
| Kurzbeschreibung (Abstract): | This paper discusses microwave-induced electroporation as a promising alternative to conventional transfection methods. Adherent C2C12 mouse cells are successfully transfected with a 5TAMRA redlabeled peptide by using a recently developed planar microwave electroporation tool. It allows to monitor the uptake kinetics with live-cell confocal microscopy and is suitable to culture, manipulate, and observe the adherent cells over several days. Viability tests with the Calcein blue AM proof the vitality of the treated cells after 72 h. The question of whether the observed effects are temperature or field induced is tackled. For this reason, comprehensive coupled full-wave electromagnetic-thermal simulations are aligned with temperature measurements. The temperature at the position of the cells does not exceed 34 °C for an input power of 24 dBm. The corresponding electric field strength is evaluated at the position of the cells. A value of 150 V/cm is not exceeded, which is at least a factor of 10 below the field strength of the conventional electroporation. Consequently, almost no cell mortality does occur during the treatment. Comparative thermal tests without a microwave field but with a successively increased temperature up to 42 °C show no uptake. In contrast, the successful uptake follows the pattern of the microwave field although the temperature distribution is homogeneous. We rate this as evidence that the uptake is induced by the high-frequency electromagnetic field rather than the temperature. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-92966 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 600 Technik, Medizin, angewandte Wissenschaften > 600 Technik |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Institut für Mikrowellentechnik und Photonik (IMP) |
| Hinterlegungsdatum: | 13 Nov 2019 09:14 |
| Letzte Änderung: | 24 Mai 2023 11:02 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Microwave Induced Electroporation of Adherent Mammalian Cells at 18 GHz. (deposited 13 Nov 2019 09:14) [Gegenwärtig angezeigt]
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