Huang, Zixin ; Loewer, Alexander (2022)
Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling.
In: TGF-Beta Signaling : Methods and Protocols
doi: 10.1007/978-1-0716-2277-3_7
Buchkapitel, Bibliographie
Kurzbeschreibung (Abstract)
Genome engineering provides a powerful tool to explore TGF-β/SMAD signaling by enabling the deletion and modification of critical components of the pathway. Over the past years, CRISPR-Cas9 technology has matured and can now be used to routinely generate knockout cell lines. Here, we describe a method to design and generate deletions of genes from the SMAD pathway in somatic human cell lines based on homologous recombination.
| Typ des Eintrags: | Buchkapitel |
|---|---|
| Erschienen: | 2022 |
| Autor(en): | Huang, Zixin ; Loewer, Alexander |
| Art des Eintrags: | Bibliographie |
| Titel: | Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling |
| Sprache: | Englisch |
| Publikationsjahr: | 2022 |
| Ort: | New York |
| Verlag: | Humana Press |
| Buchtitel: | TGF-Beta Signaling : Methods and Protocols |
| Reihe: | Methods in molecular biology (Clifton, N.J.) |
| DOI: | 10.1007/978-1-0716-2277-3_7 |
| Kurzbeschreibung (Abstract): | Genome engineering provides a powerful tool to explore TGF-β/SMAD signaling by enabling the deletion and modification of critical components of the pathway. Over the past years, CRISPR-Cas9 technology has matured and can now be used to routinely generate knockout cell lines. Here, we describe a method to design and generate deletions of genes from the SMAD pathway in somatic human cell lines based on homologous recombination. |
| ID-Nummer: | pmid:35347684 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Systems Biology of the Stress Response |
| Hinterlegungsdatum: | 05 Apr 2022 07:06 |
| Letzte Änderung: | 05 Apr 2022 07:11 |
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