Stanbekova, Gulshan ; Beisenov, Daniyar ; Nizkorodova, Anna ; Iskakov, Bulat ; Warzecha, Heribert (2021)
Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts.
In: Biotechnology letters, 43 (7)
doi: 10.1007/s10529-021-03117-x
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
OBJECTIVE
A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox.
RESULTS
Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g (FW) The serum of a sheep infected with the virus recognised the chloroplast-produced protein indicating that the protein retains its antigenic properties.
CONCLUSIONS
These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Stanbekova, Gulshan ; Beisenov, Daniyar ; Nizkorodova, Anna ; Iskakov, Bulat ; Warzecha, Heribert |
| Art des Eintrags: | Bibliographie |
| Titel: | Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts. |
| Sprache: | Englisch |
| Publikationsjahr: | Juli 2021 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biotechnology letters |
| Jahrgang/Volume einer Zeitschrift: | 43 |
| (Heft-)Nummer: | 7 |
| DOI: | 10.1007/s10529-021-03117-x |
| Kurzbeschreibung (Abstract): | OBJECTIVE A chloroplast transgenic approach was assessed in order to produce a structural protein SPPV117 of sheep pox virus in Nicotiana tabacum for the future development of a plant-based subunit vaccine against sheep pox. RESULTS Two DNA constructs containing SPPV117 coding sequence under the control of chloroplast promoter and terminator of psbA gene or rrn promoter and rbcL terminator were designed and inserted into the chloroplast genome by a biolistic method. The transgenic plants were selected via PCR analysis. Northern and Western blot analysis showed expression of the transgene at transcriptional and translational levels, respectively. The recombinant protein accumulated to about 0.3% and 0.9% of total soluble protein in leaves when expressed from psbA and rrn promoter, respectively. Plant-produced SPPV117 protein was purified using metal affinity chromatography and the protein yield was 19.67 ± 1.25 µg g (FW) The serum of a sheep infected with the virus recognised the chloroplast-produced protein indicating that the protein retains its antigenic properties. CONCLUSIONS These results demonstrate that chloroplasts are a suitable system for the production of a candidate subunit vaccine against sheep pox. |
| ID-Nummer: | pmid:33797655 |
| Zusätzliche Informationen: | ePub April 2021 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Biotechnology and Metabolic Engineering |
| Hinterlegungsdatum: | 06 Apr 2021 06:27 |
| Letzte Änderung: | 20 Dez 2021 13:04 |
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