Engel, Anja J. ; Winterstein, Laura-Marie ; Kithil, Marina ; Langhans, Markus ; Moroni, Anna ; Thiel, Gerhard (2021)
Light-Regulated Transcription of a Mitochondrial-Targeted K⁺ Channel.
In: Cells, 2020, 9 (11)
doi: 10.26083/tuprints-00019269
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
The inner membranes of mitochondria contain several types of K⁺ channels, which modulate the membrane potential of the organelle and contribute in this way to cytoprotection and the regulation of cell death. To better study the causal relationship between K⁺ channel activity and physiological changes, we developed an optogenetic platform for a light-triggered modulation of K⁺ conductance in mitochondria. By using the light-sensitive interaction between cryptochrome 2 and the regulatory protein CIB1, we can trigger the transcription of a small and highly selective K⁺ channel, which is in mammalian cells targeted into the inner membrane of mitochondria. After exposing cells to very low intensities (≤0.16 mW/mm²) of blue light, the channel protein is detectable as an accumulation of its green fluorescent protein (GFP) tag in the mitochondria less than 1 h after stimulation. This system allows for an in vivo monitoring of crucial physiological parameters of mitochondria, showing that the presence of an active K⁺ channel causes a substantial depolarization compatible with the effect of an uncoupler. Elevated K⁺ conductance also results in a decrease in the Ca²⁺ concentration in the mitochondria but has no impact on apoptosis.
Typ des Eintrags: | Artikel |
---|---|
Erschienen: | 2021 |
Autor(en): | Engel, Anja J. ; Winterstein, Laura-Marie ; Kithil, Marina ; Langhans, Markus ; Moroni, Anna ; Thiel, Gerhard |
Art des Eintrags: | Zweitveröffentlichung |
Titel: | Light-Regulated Transcription of a Mitochondrial-Targeted K⁺ Channel |
Sprache: | Englisch |
Publikationsjahr: | 2021 |
Publikationsdatum der Erstveröffentlichung: | 2020 |
Verlag: | MDPI |
Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cells |
Jahrgang/Volume einer Zeitschrift: | 9 |
(Heft-)Nummer: | 11 |
Kollation: | 16 Seiten |
DOI: | 10.26083/tuprints-00019269 |
URL / URN: | https://tuprints.ulb.tu-darmstadt.de/19269 |
Zugehörige Links: | |
Herkunft: | Zweitveröffentlichung aus gefördertem Golden Open Access |
Kurzbeschreibung (Abstract): | The inner membranes of mitochondria contain several types of K⁺ channels, which modulate the membrane potential of the organelle and contribute in this way to cytoprotection and the regulation of cell death. To better study the causal relationship between K⁺ channel activity and physiological changes, we developed an optogenetic platform for a light-triggered modulation of K⁺ conductance in mitochondria. By using the light-sensitive interaction between cryptochrome 2 and the regulatory protein CIB1, we can trigger the transcription of a small and highly selective K⁺ channel, which is in mammalian cells targeted into the inner membrane of mitochondria. After exposing cells to very low intensities (≤0.16 mW/mm²) of blue light, the channel protein is detectable as an accumulation of its green fluorescent protein (GFP) tag in the mitochondria less than 1 h after stimulation. This system allows for an in vivo monitoring of crucial physiological parameters of mitochondria, showing that the presence of an active K⁺ channel causes a substantial depolarization compatible with the effect of an uncoupler. Elevated K⁺ conductance also results in a decrease in the Ca²⁺ concentration in the mitochondria but has no impact on apoptosis. |
Status: | Verlagsversion |
URN: | urn:nbn:de:tuda-tuprints-192690 |
Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
Hinterlegungsdatum: | 09 Aug 2021 07:46 |
Letzte Änderung: | 17 Aug 2021 06:38 |
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