TU Darmstadt / ULB / TUbiblio

Light-Regulated Transcription of a Mitochondrial-Targeted K⁺ Channel

Engel, Anja J. ; Winterstein, Laura-Marie ; Kithil, Marina ; Langhans, Markus ; Moroni, Anna ; Thiel, Gerhard (2021)
Light-Regulated Transcription of a Mitochondrial-Targeted K⁺ Channel.
In: Cells, 2020, 9 (11)
doi: 10.26083/tuprints-00019269
Article, Secondary publication, Publisher's Version

Abstract

The inner membranes of mitochondria contain several types of K⁺ channels, which modulate the membrane potential of the organelle and contribute in this way to cytoprotection and the regulation of cell death. To better study the causal relationship between K⁺ channel activity and physiological changes, we developed an optogenetic platform for a light-triggered modulation of K⁺ conductance in mitochondria. By using the light-sensitive interaction between cryptochrome 2 and the regulatory protein CIB1, we can trigger the transcription of a small and highly selective K⁺ channel, which is in mammalian cells targeted into the inner membrane of mitochondria. After exposing cells to very low intensities (≤0.16 mW/mm²) of blue light, the channel protein is detectable as an accumulation of its green fluorescent protein (GFP) tag in the mitochondria less than 1 h after stimulation. This system allows for an in vivo monitoring of crucial physiological parameters of mitochondria, showing that the presence of an active K⁺ channel causes a substantial depolarization compatible with the effect of an uncoupler. Elevated K⁺ conductance also results in a decrease in the Ca²⁺ concentration in the mitochondria but has no impact on apoptosis.

Item Type: Article
Erschienen: 2021
Creators: Engel, Anja J. ; Winterstein, Laura-Marie ; Kithil, Marina ; Langhans, Markus ; Moroni, Anna ; Thiel, Gerhard
Type of entry: Secondary publication
Title: Light-Regulated Transcription of a Mitochondrial-Targeted K⁺ Channel
Language: English
Date: 2021
Year of primary publication: 2020
Publisher: MDPI
Journal or Publication Title: Cells
Volume of the journal: 9
Issue Number: 11
Collation: 16 Seiten
DOI: 10.26083/tuprints-00019269
URL / URN: https://tuprints.ulb.tu-darmstadt.de/19269
Corresponding Links:
Origin: Secondary publication via sponsored Golden Open Access
Abstract:

The inner membranes of mitochondria contain several types of K⁺ channels, which modulate the membrane potential of the organelle and contribute in this way to cytoprotection and the regulation of cell death. To better study the causal relationship between K⁺ channel activity and physiological changes, we developed an optogenetic platform for a light-triggered modulation of K⁺ conductance in mitochondria. By using the light-sensitive interaction between cryptochrome 2 and the regulatory protein CIB1, we can trigger the transcription of a small and highly selective K⁺ channel, which is in mammalian cells targeted into the inner membrane of mitochondria. After exposing cells to very low intensities (≤0.16 mW/mm²) of blue light, the channel protein is detectable as an accumulation of its green fluorescent protein (GFP) tag in the mitochondria less than 1 h after stimulation. This system allows for an in vivo monitoring of crucial physiological parameters of mitochondria, showing that the presence of an active K⁺ channel causes a substantial depolarization compatible with the effect of an uncoupler. Elevated K⁺ conductance also results in a decrease in the Ca²⁺ concentration in the mitochondria but has no impact on apoptosis.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-192690
Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
Date Deposited: 09 Aug 2021 07:46
Last Modified: 17 Aug 2021 06:38
PPN:
Corresponding Links:
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details