Engel, Anja J. ; Winterstein, Laura-Marie ; Kithil, Marina ; Langhans, Markus ; Moroni, Anna ; Thiel, Gerhard (2020)
Light-Regulated Transcription of a Mitochondrial-Targeted K+ Channel.
In: Cells, 9 (11)
doi: 10.3390/cells9112507
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The inner membranes of mitochondria contain several types of K channels, which modulate the membrane potential of the organelle and contribute in this way to cytoprotection and the regulation of cell death. To better study the causal relationship between K channel activity and physiological changes, we developed an optogenetic platform for a light-triggered modulation of K conductance in mitochondria. By using the light-sensitive interaction between cryptochrome 2 and the regulatory protein CIB1, we can trigger the transcription of a small and highly selective K channel, which is in mammalian cells targeted into the inner membrane of mitochondria. After exposing cells to very low intensities (≤0.16 mW/mm) of blue light, the channel protein is detectable as an accumulation of its green fluorescent protein (GFP) tag in the mitochondria less than 1 h after stimulation. This system allows for an in vivo monitoring of crucial physiological parameters of mitochondria, showing that the presence of an active K channel causes a substantial depolarization compatible with the effect of an uncoupler. Elevated K conductance also results in a decrease in the Ca concentration in the mitochondria but has no impact on apoptosis.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Engel, Anja J. ; Winterstein, Laura-Marie ; Kithil, Marina ; Langhans, Markus ; Moroni, Anna ; Thiel, Gerhard |
| Art des Eintrags: | Bibliographie |
| Titel: | Light-Regulated Transcription of a Mitochondrial-Targeted K+ Channel. |
| Sprache: | Englisch |
| Publikationsjahr: | 19 November 2020 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cells |
| Jahrgang/Volume einer Zeitschrift: | 9 |
| (Heft-)Nummer: | 11 |
| DOI: | 10.3390/cells9112507 |
| URL / URN: | https://www.mdpi.com/2073-4409/9/11/2507 |
| Kurzbeschreibung (Abstract): | The inner membranes of mitochondria contain several types of K channels, which modulate the membrane potential of the organelle and contribute in this way to cytoprotection and the regulation of cell death. To better study the causal relationship between K channel activity and physiological changes, we developed an optogenetic platform for a light-triggered modulation of K conductance in mitochondria. By using the light-sensitive interaction between cryptochrome 2 and the regulatory protein CIB1, we can trigger the transcription of a small and highly selective K channel, which is in mammalian cells targeted into the inner membrane of mitochondria. After exposing cells to very low intensities (≤0.16 mW/mm) of blue light, the channel protein is detectable as an accumulation of its green fluorescent protein (GFP) tag in the mitochondria less than 1 h after stimulation. This system allows for an in vivo monitoring of crucial physiological parameters of mitochondria, showing that the presence of an active K channel causes a substantial depolarization compatible with the effect of an uncoupler. Elevated K conductance also results in a decrease in the Ca concentration in the mitochondria but has no impact on apoptosis. |
| ID-Nummer: | pmid:33228123 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 30 Nov 2020 12:44 |
| Letzte Änderung: | 30 Nov 2020 12:44 |
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