Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald (2019):
Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
In: Chemistry (Weinheim an der Bergstrasse, Germany), 25 (7), pp. 1746-1751. ISSN 1521-3765,
DOI: 10.1002/chem.201804889,
[Article]
Abstract
Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.
| Item Type: | Article |
|---|---|
| Erschienen: | 2019 |
| Creators: | Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald |
| Title: | Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers. |
| Language: | English |
| Abstract: | Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics. |
| Journal or Publication Title: | Chemistry (Weinheim an der Bergstrasse, Germany) |
| Volume of the journal: | 25 |
| Issue Number: | 7 |
| Divisions: | 10 Department of Biology 10 Department of Biology > Membrane Dynamics |
| Date Deposited: | 13 Nov 2018 07:50 |
| DOI: | 10.1002/chem.201804889 |
| URL / URN: | https://doi.org/10.1002/chem.201804889 |
| Identification Number: | pmid:30395364 |
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