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Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.

Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald (2019)
Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
In: Chemistry (Weinheim an der Bergstrasse, Germany), 25 (7)
doi: 10.1002/chem.201804889
Article, Bibliographie

Abstract

Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.

Item Type: Article
Erschienen: 2019
Creators: Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald
Type of entry: Bibliographie
Title: Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
Language: English
Date: 1 February 2019
Journal or Publication Title: Chemistry (Weinheim an der Bergstrasse, Germany)
Volume of the journal: 25
Issue Number: 7
DOI: 10.1002/chem.201804889
URL / URN: https://doi.org/10.1002/chem.201804889
Abstract:

Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.

Identification Number: pmid:30395364
Divisions: 10 Department of Biology
10 Department of Biology > Membrane Dynamics
Date Deposited: 13 Nov 2018 07:50
Last Modified: 11 Feb 2019 13:27
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