TU Darmstadt / ULB / TUbiblio

A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement.

Thomsen, Andreas R. and Aldrian, Christine and Bronsert, Peter and Thomann, Yi and Nanko, Norbert and Melin, Nicolas and Rücker, Gerta and Follo, Marie and Grosu, Anca L. and Niedermann, Gabriele and Layer, Paul G. and Heselich, Anja and Lund, Per G. (2018):
A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement.
In: Lab on a chip, 18 (1), pp. 179-189, ISSN 1473-0189,
[Article]

Abstract

Multicellular spheroids represent a well-established 3D model to study healthy and diseased cells in vitro. The use of conventional 3D cell culture platforms for the generation of multicellular spheroids is limited to cell types that easily self-assemble into spheroids because less adhesive cells fail to form stable aggregates. A high-precision micromoulding technique developed in our laboratory produces deep conical agarose microwell arrays that allow the cultivation of uniform multicellular aggregates, irrespective of the spheroid formation capacity of the cells. Such hydrogel arrays warrant a steady nutrient supply for several weeks, permit live volumetric measurements to monitor cell growth, enable immunohistochemical staining, fluorescence-based microscopy, and facilitate immediate harvesting of cell aggregates. This system also allows co-cultures of two distinct cell types either in direct cell-cell contact or at a distance as the hydrogel permits diffusion of soluble compounds. Notably, we show that co-culture of a breast cancer cell line with bone marrow stromal cells enhances 3D growth of the cancer cells in this system.

Item Type: Article
Erschienen: 2018
Creators: Thomsen, Andreas R. and Aldrian, Christine and Bronsert, Peter and Thomann, Yi and Nanko, Norbert and Melin, Nicolas and Rücker, Gerta and Follo, Marie and Grosu, Anca L. and Niedermann, Gabriele and Layer, Paul G. and Heselich, Anja and Lund, Per G.
Title: A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement.
Language: English
Abstract:

Multicellular spheroids represent a well-established 3D model to study healthy and diseased cells in vitro. The use of conventional 3D cell culture platforms for the generation of multicellular spheroids is limited to cell types that easily self-assemble into spheroids because less adhesive cells fail to form stable aggregates. A high-precision micromoulding technique developed in our laboratory produces deep conical agarose microwell arrays that allow the cultivation of uniform multicellular aggregates, irrespective of the spheroid formation capacity of the cells. Such hydrogel arrays warrant a steady nutrient supply for several weeks, permit live volumetric measurements to monitor cell growth, enable immunohistochemical staining, fluorescence-based microscopy, and facilitate immediate harvesting of cell aggregates. This system also allows co-cultures of two distinct cell types either in direct cell-cell contact or at a distance as the hydrogel permits diffusion of soluble compounds. Notably, we show that co-culture of a breast cancer cell line with bone marrow stromal cells enhances 3D growth of the cancer cells in this system.

Journal or Publication Title: Lab on a chip
Volume: 18
Number: 1
Divisions: 10 Department of Biology
10 Department of Biology > Developmental Biology and Neurogenetics
Date Deposited: 11 Dec 2017 13:18
Identification Number: pmid:29211089
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)

View Item View Item