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The iNAPO Project: Biomimetic Nanopores for a New Generation of Lab-on-Chip Micro Sensors

Ensinger, Wolfgang and Ali, Mubarak and Nasir, Saima and Duznovic, Ivana and Trautmann, Christina and Toimil-Molares, Maria Eugenia and Distefano, Giuseppa R. and Laube, Bodo and Bernhard, Max and Mikosch-Wersching, Melanie and Schlaak, Helmut F. and El Khoury, Mario (2017):
The iNAPO Project: Biomimetic Nanopores for a New Generation of Lab-on-Chip Micro Sensors.
In: Proceedings of the 2nd World Congress on Recent Advances in Nanotechnology, In: 2nd World Congress on Recent Advances in Nanotechnology (RAN’17), Barcelona, Spanien, 4. - 6. April 2017, ISSN 2371-5308, DOI: 10.11159/icnnfc17.141,
[Online-Edition: https://doi.org/10.11159/icnnfc17.141],
[Conference or Workshop Item]

Abstract

In nature, ion conducting nanopores play a vital role for the function of living cells. They undergo gating processes where they open and close upon an external stimulus, such as the presence of a particular biomolecule, the ligand. When the gating process is observed and is quantitatively measured one can derive data about the presence and the amount of the ligand. Hence, the nanopores can be utilized for specific sensing. However, biological nanopores are embedded in a biological cell membrane that is fragile and unstable with respect to storage and application. The iNAPO project aims at combining robust polymer-based nanopores with protein-based biological nanopores, thus combining the selectivity and sensitivity of the latter with the stability and processability of the first ones. This paper describes the different steps in the fabrication of ion conducting nanopores. It begins with ion irradiation of polymer foils, combined with chemical etching of the ion damage tracks into nanopores. By means of chemical coupling reactions, the nanopore walls are functionalized with particular molecules which react or bioconjugate with the molecules to be analyzed. As an example, a recent result on sensing a physiologically active P-based anion is shown. By means of a complexation reaction with Zn-di(picolyl)amine, the selective measurement of the concentration of the anion pyrophosphate is demonstrated. In the final step of the project, the nanopores will be incorporated into a Lab-on-Chip system for applications in e.g. medical diagnostics and environmental analysis.

Item Type: Conference or Workshop Item
Erschienen: 2017
Creators: Ensinger, Wolfgang and Ali, Mubarak and Nasir, Saima and Duznovic, Ivana and Trautmann, Christina and Toimil-Molares, Maria Eugenia and Distefano, Giuseppa R. and Laube, Bodo and Bernhard, Max and Mikosch-Wersching, Melanie and Schlaak, Helmut F. and El Khoury, Mario
Title: The iNAPO Project: Biomimetic Nanopores for a New Generation of Lab-on-Chip Micro Sensors
Language: English
Abstract:

In nature, ion conducting nanopores play a vital role for the function of living cells. They undergo gating processes where they open and close upon an external stimulus, such as the presence of a particular biomolecule, the ligand. When the gating process is observed and is quantitatively measured one can derive data about the presence and the amount of the ligand. Hence, the nanopores can be utilized for specific sensing. However, biological nanopores are embedded in a biological cell membrane that is fragile and unstable with respect to storage and application. The iNAPO project aims at combining robust polymer-based nanopores with protein-based biological nanopores, thus combining the selectivity and sensitivity of the latter with the stability and processability of the first ones. This paper describes the different steps in the fabrication of ion conducting nanopores. It begins with ion irradiation of polymer foils, combined with chemical etching of the ion damage tracks into nanopores. By means of chemical coupling reactions, the nanopore walls are functionalized with particular molecules which react or bioconjugate with the molecules to be analyzed. As an example, a recent result on sensing a physiologically active P-based anion is shown. By means of a complexation reaction with Zn-di(picolyl)amine, the selective measurement of the concentration of the anion pyrophosphate is demonstrated. In the final step of the project, the nanopores will be incorporated into a Lab-on-Chip system for applications in e.g. medical diagnostics and environmental analysis.

Title of Book: Proceedings of the 2nd World Congress on Recent Advances in Nanotechnology
Uncontrolled Keywords: ion track etching, polymer membrane, nanopores, biomimetic sensor, nanosensor, current-potential measurements
Divisions: 11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Material Analytics
Event Title: 2nd World Congress on Recent Advances in Nanotechnology (RAN’17)
Event Location: Barcelona, Spanien
Event Dates: 4. - 6. April 2017
Date Deposited: 21 Jul 2017 09:21
DOI: 10.11159/icnnfc17.141
Official URL: https://doi.org/10.11159/icnnfc17.141
Funders: This work has been supported in the frame of the LOEWE project iNAPO by the Hessen State Ministry of Higher Education, Research and the Arts.
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