Wieneke, Ralph ; Laboria, Noemi ; Rajan, Malini ; Kollmannsperger, Alina ; Natale, Francesco ; Cardoso, M. Cristina ; Tampé, Robert (2014)
Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.
In: Journal of the American Chemical Society, 136 (40)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The selective and fast labeling of proteins in living cells is a major challenge. Live-cell labeling techniques require a high specificity, high labeling density, and cell permeability of the tagging molecule to target the protein of interest. Here, we report on the site-specific, rapid and efficient labeling of endogenous and recombinant histidine-tagged proteins at distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes. In vivo labeling was followed in real-time in living cells, demonstrating a high specificity and high degree of colocalization in the crowded cellular environment.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2014 |
| Autor(en): | Wieneke, Ralph ; Laboria, Noemi ; Rajan, Malini ; Kollmannsperger, Alina ; Natale, Francesco ; Cardoso, M. Cristina ; Tampé, Robert |
| Art des Eintrags: | Bibliographie |
| Titel: | Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes. |
| Sprache: | Englisch |
| Publikationsjahr: | 2014 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of the American Chemical Society |
| Jahrgang/Volume einer Zeitschrift: | 136 |
| (Heft-)Nummer: | 40 |
| Kurzbeschreibung (Abstract): | The selective and fast labeling of proteins in living cells is a major challenge. Live-cell labeling techniques require a high specificity, high labeling density, and cell permeability of the tagging molecule to target the protein of interest. Here, we report on the site-specific, rapid and efficient labeling of endogenous and recombinant histidine-tagged proteins at distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes. In vivo labeling was followed in real-time in living cells, demonstrating a high specificity and high degree of colocalization in the crowded cellular environment. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 13 Nov 2014 08:05 |
| Letzte Änderung: | 13 Nov 2014 08:05 |
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