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Spatiotemporal visualization of DNA replication dynamics.

Reinhart, Marius and Casas-Delucchi, Corella S. and Cardoso, M. Cristina (2013):
Spatiotemporal visualization of DNA replication dynamics.
In: Methods in molecular biology (Clifton, N.J.), pp. 213-25, 1042, ISSN 1940-6029,
[Article]

Abstract

The ability of cells to copy their DNA allows them to transmit their genetic information to their progeny. In such, this central biological process preserves the instructions that direct the entire development of a cell. Earlier biochemical analysis in vitro and genetic analysis in yeast laid the basis of our understanding of the highly conserved mechanism of DNA replication. Recent advances on labeling and live-cell microscopy permit now the dissection of this fundamental process in vivo within the context of intact cells. In this chapter, we describe in detail how to perform multiple DNA replication labeling and detection allowing high spatial resolution imaging, as well as how to follow DNA replication in living cells allowing high temporal resolution imaging.

Item Type: Article
Erschienen: 2013
Creators: Reinhart, Marius and Casas-Delucchi, Corella S. and Cardoso, M. Cristina
Title: Spatiotemporal visualization of DNA replication dynamics.
Language: English
Abstract:

The ability of cells to copy their DNA allows them to transmit their genetic information to their progeny. In such, this central biological process preserves the instructions that direct the entire development of a cell. Earlier biochemical analysis in vitro and genetic analysis in yeast laid the basis of our understanding of the highly conserved mechanism of DNA replication. Recent advances on labeling and live-cell microscopy permit now the dissection of this fundamental process in vivo within the context of intact cells. In this chapter, we describe in detail how to perform multiple DNA replication labeling and detection allowing high spatial resolution imaging, as well as how to follow DNA replication in living cells allowing high temporal resolution imaging.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Volume: 1042
Divisions: 10 Department of Biology
10 Department of Biology > Cell Biology and Epigenetics
Date Deposited: 03 Sep 2013 11:04
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