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New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions.

Herce, H. D. and Casas-Delucchi, C. S. and Cardoso, M. Cristina (2013):
New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions.
In: Journal of microscopy, 249 (3), pp. 184-194, ISSN 1365-2818,
[Article]

Abstract

The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are usually quantified by analysing the correlation and/or the overlap between images, using the Pearson's and Manders' coefficients, respectively. However, the correlation and overlap coefficients are parameters not designed to quantify molecular interactions. Here we propose a new colocalization coefficient specifically designed to quantify the interactions between molecules. In well-defined thermodynamic ensembles, this coefficient can in principle be used to calculate relevant statistical thermodynamic quantities such as binding free energies.

Item Type: Article
Erschienen: 2013
Creators: Herce, H. D. and Casas-Delucchi, C. S. and Cardoso, M. Cristina
Title: New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions.
Language: English
Abstract:

The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are usually quantified by analysing the correlation and/or the overlap between images, using the Pearson's and Manders' coefficients, respectively. However, the correlation and overlap coefficients are parameters not designed to quantify molecular interactions. Here we propose a new colocalization coefficient specifically designed to quantify the interactions between molecules. In well-defined thermodynamic ensembles, this coefficient can in principle be used to calculate relevant statistical thermodynamic quantities such as binding free energies.

Journal or Publication Title: Journal of microscopy
Volume: 249
Number: 3
Divisions: 10 Department of Biology
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10 Department of Biology > Cell Biology and Epigenetics
Date Deposited: 14 Jan 2013 13:04
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