Herce, H. D. ; Casas-Delucchi, C. S. ; Cardoso, M. Cristina (2013)
New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions.
In: Journal of microscopy, 249 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are usually quantified by analysing the correlation and/or the overlap between images, using the Pearson's and Manders' coefficients, respectively. However, the correlation and overlap coefficients are parameters not designed to quantify molecular interactions. Here we propose a new colocalization coefficient specifically designed to quantify the interactions between molecules. In well-defined thermodynamic ensembles, this coefficient can in principle be used to calculate relevant statistical thermodynamic quantities such as binding free energies.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2013 |
| Autor(en): | Herce, H. D. ; Casas-Delucchi, C. S. ; Cardoso, M. Cristina |
| Art des Eintrags: | Bibliographie |
| Titel: | New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions. |
| Sprache: | Englisch |
| Publikationsjahr: | 2013 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of microscopy |
| Jahrgang/Volume einer Zeitschrift: | 249 |
| (Heft-)Nummer: | 3 |
| Kurzbeschreibung (Abstract): | The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are usually quantified by analysing the correlation and/or the overlap between images, using the Pearson's and Manders' coefficients, respectively. However, the correlation and overlap coefficients are parameters not designed to quantify molecular interactions. Here we propose a new colocalization coefficient specifically designed to quantify the interactions between molecules. In well-defined thermodynamic ensembles, this coefficient can in principle be used to calculate relevant statistical thermodynamic quantities such as binding free energies. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 14 Jan 2013 13:04 |
| Letzte Änderung: | 26 Mär 2013 10:10 |
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