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Phycodnavirus potassium ion channel proteins question the virus molecular piracy hypothesis.

Hamacher, Kay and Greiner, Timo and Ogata, Hiroyuki and Van Etten, James L. and Gebhardt, Manuela and Villarreal, Luis P. and Cosentino, Cristian and Moroni, Anna and Thiel, Gerhard :
Phycodnavirus potassium ion channel proteins question the virus molecular piracy hypothesis.
[Online-Edition: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjourna...]
In: PloS one, 7 (6) e38826. ISSN 1932-6203
[Article] , (2012)

Official URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjourna...

Abstract

Phycodnaviruses are large dsDNA, algal-infecting viruses that encode many genes with homologs in prokaryotes and eukaryotes. Among the viral gene products are the smallest proteins known to form functional K(+) channels. To determine if these viral K(+) channels are the product of molecular piracy from their hosts, we compared the sequences of the K(+) channel pore modules from seven phycodnaviruses to the K(+) channels from Chlorella variabilis and Ectocarpus siliculosus, whose genomes have recently been sequenced. C. variabilis is the host for two of the viruses PBCV-1 and NY-2A and E. siliculosus is the host for the virus EsV-1. Systematic phylogenetic analyses consistently indicate that the viral K(+) channels are not related to any lineage of the host channel homologs and that they are more closely related to each other than to their host homologs. A consensus sequence of the viral channels resembles a protein of unknown function from a proteobacterium. However, the bacterial protein lacks the consensus motif of all K(+) channels and it does not form a functional channel in yeast, suggesting that the viral channels did not come from a proteobacterium. Collectively, our results indicate that the viruses did not acquire their K(+) channel-encoding genes from their current algal hosts by gene transfer; thus alternative explanations are required. One possibility is that the viral genes arose from ancient organisms, which served as their hosts before the viruses developed their current host specificity. Alternatively the viral proteins could be the origin of K(+) channels in algae and perhaps even all cellular organisms.

Item Type: Article
Erschienen: 2012
Creators: Hamacher, Kay and Greiner, Timo and Ogata, Hiroyuki and Van Etten, James L. and Gebhardt, Manuela and Villarreal, Luis P. and Cosentino, Cristian and Moroni, Anna and Thiel, Gerhard
Title: Phycodnavirus potassium ion channel proteins question the virus molecular piracy hypothesis.
Language: English
Abstract:

Phycodnaviruses are large dsDNA, algal-infecting viruses that encode many genes with homologs in prokaryotes and eukaryotes. Among the viral gene products are the smallest proteins known to form functional K(+) channels. To determine if these viral K(+) channels are the product of molecular piracy from their hosts, we compared the sequences of the K(+) channel pore modules from seven phycodnaviruses to the K(+) channels from Chlorella variabilis and Ectocarpus siliculosus, whose genomes have recently been sequenced. C. variabilis is the host for two of the viruses PBCV-1 and NY-2A and E. siliculosus is the host for the virus EsV-1. Systematic phylogenetic analyses consistently indicate that the viral K(+) channels are not related to any lineage of the host channel homologs and that they are more closely related to each other than to their host homologs. A consensus sequence of the viral channels resembles a protein of unknown function from a proteobacterium. However, the bacterial protein lacks the consensus motif of all K(+) channels and it does not form a functional channel in yeast, suggesting that the viral channels did not come from a proteobacterium. Collectively, our results indicate that the viruses did not acquire their K(+) channel-encoding genes from their current algal hosts by gene transfer; thus alternative explanations are required. One possibility is that the viral genes arose from ancient organisms, which served as their hosts before the viruses developed their current host specificity. Alternatively the viral proteins could be the origin of K(+) channels in algae and perhaps even all cellular organisms.

Journal or Publication Title: PloS one
Volume: 7
Number: 6
Divisions: 10 Department of Biology > Plant Membrane Biophysics
10 Department of Biology > Computational Biology and Simulation
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10 Department of Biology
Date Deposited: 19 Jun 2012 12:50
Official URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjourna...
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