Uehlein, Norbert ; Sperling, Henrik ; Heckwolf, Marlies ; Kaldenhoff, Ralf (2012)
The Arabidopsis aquaporin PIP1;2 rules cellular CO(2) uptake.
In: Plant, cell & environment, 35 (6)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The membrane CO(2) flux into Arabidopsis mesophyll cells was studied using a scanning pH microelectrode. Arabidopsis thaliana mesophyll cells were exposed to photosynthesis-triggering light intensities, which induced cellular CO(2) uptake. Data obtained on a AtPIP1;2 T-DNA insertion line indicated that under these conditions, cellular CO(2) transport was not limited by unstirred layer effects but was dependent on the expression of the aquaporin AtPIP1;2. Complementation of the AtPIP1;2 knockout restored membrane CO(2) transport levels to that of controls. The results provide new arguments for the ongoing debate about the validity of the lipid bilayer model system and the Meyer-Overton rule for cellular gas transport. In conclusion, we suggest a modified model of molecular gas transport mechanisms in living cells. © 2011 Blackwell Publishing Ltd.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Uehlein, Norbert ; Sperling, Henrik ; Heckwolf, Marlies ; Kaldenhoff, Ralf |
| Art des Eintrags: | Bibliographie |
| Titel: | The Arabidopsis aquaporin PIP1;2 rules cellular CO(2) uptake. |
| Sprache: | Englisch |
| Publikationsjahr: | 2012 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Plant, cell & environment |
| Jahrgang/Volume einer Zeitschrift: | 35 |
| (Heft-)Nummer: | 6 |
| Kurzbeschreibung (Abstract): | The membrane CO(2) flux into Arabidopsis mesophyll cells was studied using a scanning pH microelectrode. Arabidopsis thaliana mesophyll cells were exposed to photosynthesis-triggering light intensities, which induced cellular CO(2) uptake. Data obtained on a AtPIP1;2 T-DNA insertion line indicated that under these conditions, cellular CO(2) transport was not limited by unstirred layer effects but was dependent on the expression of the aquaporin AtPIP1;2. Complementation of the AtPIP1;2 knockout restored membrane CO(2) transport levels to that of controls. The results provide new arguments for the ongoing debate about the validity of the lipid bilayer model system and the Meyer-Overton rule for cellular gas transport. In conclusion, we suggest a modified model of molecular gas transport mechanisms in living cells. © 2011 Blackwell Publishing Ltd. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Applied Plant Sciences ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 21 Dez 2011 13:44 |
| Letzte Änderung: | 05 Mär 2013 09:57 |
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