Layer, Paul G. ; Vollmer, G. ; Kotz, S. (1983)
Selective uptake of Lucifer Yellow into different cell populations of the developing chicken retina.
In: Neuroscience letters, 35 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Whole chick embryo eyes at different stages of development were incubated in a solution of Lucifer Yellow (LY) and examined in frozen sections. Different cell populations selectively take up the dye during the course of development: from embryonic day 4 - day 8 of incubation (E4-E8) only a subpopulation of neuroepithelial cells are stained, which at E7-E8 are located mainly near the periphery between the prospective ora serrata and the outer edge of the retina. This staining disappears after E8. A second phase starts at E10, with a weak but specific staining of a small population of cells in the outer nuclear layer which becomes less pronounced by E12. Starting around E10.5 a bright staining of cells in the innermost part of the inner nuclear layer can be observed. Their size, shape and location suggest that most of them represent a subclass of amacrine cells (including all 'displaced amacrine cells'). Beginning at E15, the staining pattern becomes more complex. A single cell analysis of the percentage of LY-stained cells which are stained either before or after papain-treatment and dissociation of the intact tissue confirms and extends the histological findings.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1983 |
| Autor(en): | Layer, Paul G. ; Vollmer, G. ; Kotz, S. |
| Art des Eintrags: | Bibliographie |
| Titel: | Selective uptake of Lucifer Yellow into different cell populations of the developing chicken retina. |
| Sprache: | Englisch |
| Publikationsjahr: | 1983 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Neuroscience letters |
| Jahrgang/Volume einer Zeitschrift: | 35 |
| (Heft-)Nummer: | 3 |
| Kurzbeschreibung (Abstract): | Whole chick embryo eyes at different stages of development were incubated in a solution of Lucifer Yellow (LY) and examined in frozen sections. Different cell populations selectively take up the dye during the course of development: from embryonic day 4 - day 8 of incubation (E4-E8) only a subpopulation of neuroepithelial cells are stained, which at E7-E8 are located mainly near the periphery between the prospective ora serrata and the outer edge of the retina. This staining disappears after E8. A second phase starts at E10, with a weak but specific staining of a small population of cells in the outer nuclear layer which becomes less pronounced by E12. Starting around E10.5 a bright staining of cells in the innermost part of the inner nuclear layer can be observed. Their size, shape and location suggest that most of them represent a subclass of amacrine cells (including all 'displaced amacrine cells'). Beginning at E15, the staining pattern becomes more complex. A single cell analysis of the percentage of LY-stained cells which are stained either before or after papain-treatment and dissociation of the intact tissue confirms and extends the histological findings. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Developmental Biology and Neurogenetics |
| Hinterlegungsdatum: | 21 Nov 2011 13:20 |
| Letzte Änderung: | 05 Mär 2013 09:56 |
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