Layer, Paul G. ; Alber, R. ; Mansky, P. ; Vollmer, G. ; Willbold, E. (1990)
Regeneration of a chimeric retina from single cells in vitro: cell-lineage-dependent formation of radial cell columns by segregated chick and quail cells.
In: Cell and tissue research, 259 (2)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
We report here that similar to E6-chicken retinal cells, dissociated cells from 5.5-day-old (E5.5) quail retinae reaggregate in rotary culture, multiply about tenfold and reestablish histotypical areas. These cellular aggregates include all nuclear layers either with inversed or correct laminar polarity, depending on the local origin of the cells (called "rosetted" and "laminar" in-vitro-retinae (IVR), respectively; Layer and Willbold 1989). In combined cultures, chick and quail cells are evenly mixed only during the first two days of culture. Along with the assembly of single cells into rosettes and then into discrete laminae, sectors of chick and quail cells begin to segregate. They are delineated by borders running radially through all three nuclear layers. Thus, interspecies migration of cells at this advanced stage of differentiation is strongly inhibited. Concomitant with this segregation, coherent radial columns spanning all three layers but containing cells from either species only, can be traced histologically. We conclude that a weak segregation of chick and quail retinal cells takes place already at the single cell level, but that the permanent segregation of entire tissue parts must be due to clonal cellular proliferation within the IVR in conjunction with some developmental-structure mechanism retaining clonal progenies within a columnar order.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1990 |
| Autor(en): | Layer, Paul G. ; Alber, R. ; Mansky, P. ; Vollmer, G. ; Willbold, E. |
| Art des Eintrags: | Bibliographie |
| Titel: | Regeneration of a chimeric retina from single cells in vitro: cell-lineage-dependent formation of radial cell columns by segregated chick and quail cells. |
| Sprache: | Englisch |
| Publikationsjahr: | 1990 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cell and tissue research |
| Jahrgang/Volume einer Zeitschrift: | 259 |
| (Heft-)Nummer: | 2 |
| Kurzbeschreibung (Abstract): | We report here that similar to E6-chicken retinal cells, dissociated cells from 5.5-day-old (E5.5) quail retinae reaggregate in rotary culture, multiply about tenfold and reestablish histotypical areas. These cellular aggregates include all nuclear layers either with inversed or correct laminar polarity, depending on the local origin of the cells (called "rosetted" and "laminar" in-vitro-retinae (IVR), respectively; Layer and Willbold 1989). In combined cultures, chick and quail cells are evenly mixed only during the first two days of culture. Along with the assembly of single cells into rosettes and then into discrete laminae, sectors of chick and quail cells begin to segregate. They are delineated by borders running radially through all three nuclear layers. Thus, interspecies migration of cells at this advanced stage of differentiation is strongly inhibited. Concomitant with this segregation, coherent radial columns spanning all three layers but containing cells from either species only, can be traced histologically. We conclude that a weak segregation of chick and quail retinal cells takes place already at the single cell level, but that the permanent segregation of entire tissue parts must be due to clonal cellular proliferation within the IVR in conjunction with some developmental-structure mechanism retaining clonal progenies within a columnar order. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Developmental Biology and Neurogenetics |
| Hinterlegungsdatum: | 21 Nov 2011 12:59 |
| Letzte Änderung: | 05 Mär 2013 09:56 |
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