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Non-antisense cellular responses to oligonucleotides.

Anselmet, Alain ; Mayat, Ebrahim ; Wietek, Stefan ; Layer, Paul G. ; Payrastre, Bernard ; Massoulié, Jean :
Non-antisense cellular responses to oligonucleotides.
In: FEBS letters, 510 (3) pp. 175-80. ISSN 0014-5793
[Artikel], (2002)

Kurzbeschreibung (Abstract)

Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10-20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments.

Typ des Eintrags: Artikel
Erschienen: 2002
Autor(en): Anselmet, Alain ; Mayat, Ebrahim ; Wietek, Stefan ; Layer, Paul G. ; Payrastre, Bernard ; Massoulié, Jean
Titel: Non-antisense cellular responses to oligonucleotides.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10-20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: FEBS letters
Band: 510
(Heft-)Nummer: 3
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology
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Fachbereich Biologie, Biology > Entwicklungsbiologie und Neurogenetik, Developmental Biology and Neurogenetics
Hinterlegungsdatum: 21 Nov 2011 09:40
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