Anselmet, Alain ; Mayat, Ebrahim ; Wietek, Stefan ; Layer, Paul G. ; Payrastre, Bernard ; Massoulié, Jean (2002)
Non-antisense cellular responses to oligonucleotides.
In: FEBS letters, 510 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10-20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2002 |
| Autor(en): | Anselmet, Alain ; Mayat, Ebrahim ; Wietek, Stefan ; Layer, Paul G. ; Payrastre, Bernard ; Massoulié, Jean |
| Art des Eintrags: | Bibliographie |
| Titel: | Non-antisense cellular responses to oligonucleotides. |
| Sprache: | Englisch |
| Publikationsjahr: | 2002 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 510 |
| (Heft-)Nummer: | 3 |
| Kurzbeschreibung (Abstract): | Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10-20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Developmental Biology and Neurogenetics |
| Hinterlegungsdatum: | 21 Nov 2011 09:40 |
| Letzte Änderung: | 05 Mär 2013 09:55 |
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