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The tobacco plasma membrane aquaporin NtAQP1.

Siefritz, F. and Biela, A. and Eckert, M. and Otto, Beate and Uehlein, Norbert and Kaldenhoff, Ralf (2001):
The tobacco plasma membrane aquaporin NtAQP1.
In: Journal of experimental botany, pp. 1953-7, 52, (363), ISSN 0022-0957, [Article]

Abstract

This paper gives a summary of a project to characterize a tobacco aquaporin. The cDNA and gene, including the 5' upstream region, for the tobacco aquaporin NtAQP1 has been isolated and the encoded protein characterized. The significance of promoter regions for an abscisic acid- and gibberellic acid-induced gene expression could be restricted to a region between -1450 and -1112 upstream of the transcription start point by transient transformation of a bicistronic vector into tobacco protoplasts. NtAQP1 expression in tobacco plants was found to be elevated in flowers, stems and roots. In roots, the protein was detected close to xylem vessels in pitch-like structures. Studies with a NtAQP1-GFP fusion indicated a plasma membrane location. For a functional analysis, the cDNA was expressed in Xenopus oocytes. NtAQP1 was found to be a heavy metal-insensitive aquaporin with additional permeability for glycerol. Mutation of a threonine at position 233 to a cysteine transformed NtAQP1 into a heavy metal-sensitive aquaporin.

Item Type: Article
Erschienen: 2001
Creators: Siefritz, F. and Biela, A. and Eckert, M. and Otto, Beate and Uehlein, Norbert and Kaldenhoff, Ralf
Title: The tobacco plasma membrane aquaporin NtAQP1.
Language: English
Abstract:

This paper gives a summary of a project to characterize a tobacco aquaporin. The cDNA and gene, including the 5' upstream region, for the tobacco aquaporin NtAQP1 has been isolated and the encoded protein characterized. The significance of promoter regions for an abscisic acid- and gibberellic acid-induced gene expression could be restricted to a region between -1450 and -1112 upstream of the transcription start point by transient transformation of a bicistronic vector into tobacco protoplasts. NtAQP1 expression in tobacco plants was found to be elevated in flowers, stems and roots. In roots, the protein was detected close to xylem vessels in pitch-like structures. Studies with a NtAQP1-GFP fusion indicated a plasma membrane location. For a functional analysis, the cDNA was expressed in Xenopus oocytes. NtAQP1 was found to be a heavy metal-insensitive aquaporin with additional permeability for glycerol. Mutation of a threonine at position 233 to a cysteine transformed NtAQP1 into a heavy metal-sensitive aquaporin.

Journal or Publication Title: Journal of experimental botany
Volume: 52
Number: 363
Divisions: 10 Department of Biology > Applied Plant Sciences
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10 Department of Biology
Date Deposited: 31 Aug 2011 13:05
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