Kuefner, M. A. ; Grudzenski, S. ; Hamann, J. ; Achenbach, S. ; Lell, Michael ; Anderson, K. ; Schwab, S. A. ; Häberle, L. ; Löbrich, M. ; Uder, M. (2010)
Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography.
In: European radiology
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
AIMS: To compare in vivo DNA lesions induced during helical and sequential coronary computed tomography angiography (CTA) and to evaluate the effect of CT parameters on double-strand break (DSB) levels. METHODS: Thirty-six patients were examined with various CT protocols and modes (helical scan, n = 27; sequential scan, n = 9) either using a 64-slice dual-source or a 128-slice CT system. Blood samples were obtained before and 30 min after CT. Lymphocytes were isolated, stained against the phosphorylated histone variant gamma-H2AX, and DSBs were visualised by using fluorescence microscopy. RESULTS: DSB yields 30 min after CTA ranged from 0.04 to 0.71 per cell and showed a significant correlation to DLP (rho = 0.81, p < 0.00001). Median DSB yield and median DLP were significantly lower after sequential compared to helical CT examinations (0.11 vs. 0.37 DSBs/cell and 249 vs. 958 mGy cm, p < 0.00001). Additional calcium scoring led to an increase in DLP (p = 0.15) and DSB levels (p = 0.04). DSB levels normalised to the DLP showed a significant correlation to the attenuation of the blood (rho = 0.53, p = 0.01) and a negative correlation to the body mass index of the patients (rho = -0.37, p = 0.06). CONCLUSION: gamma-H2AX immunofluorescence microscopy allows one to determine dose-related effects on x-ray-induced DSB levels and to consider individual factors which cannot be monitored by physical dose measurements.
Typ des Eintrags: | Artikel |
---|---|
Erschienen: | 2010 |
Autor(en): | Kuefner, M. A. ; Grudzenski, S. ; Hamann, J. ; Achenbach, S. ; Lell, Michael ; Anderson, K. ; Schwab, S. A. ; Häberle, L. ; Löbrich, M. ; Uder, M. |
Art des Eintrags: | Bibliographie |
Titel: | Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography. |
Sprache: | Englisch |
Publikationsjahr: | 2010 |
Titel der Zeitschrift, Zeitung oder Schriftenreihe: | European radiology |
URL / URN: | http://www.ncbi.nlm.nih.gov/pubmed/20625737 |
Kurzbeschreibung (Abstract): | AIMS: To compare in vivo DNA lesions induced during helical and sequential coronary computed tomography angiography (CTA) and to evaluate the effect of CT parameters on double-strand break (DSB) levels. METHODS: Thirty-six patients were examined with various CT protocols and modes (helical scan, n = 27; sequential scan, n = 9) either using a 64-slice dual-source or a 128-slice CT system. Blood samples were obtained before and 30 min after CT. Lymphocytes were isolated, stained against the phosphorylated histone variant gamma-H2AX, and DSBs were visualised by using fluorescence microscopy. RESULTS: DSB yields 30 min after CTA ranged from 0.04 to 0.71 per cell and showed a significant correlation to DLP (rho = 0.81, p < 0.00001). Median DSB yield and median DLP were significantly lower after sequential compared to helical CT examinations (0.11 vs. 0.37 DSBs/cell and 249 vs. 958 mGy cm, p < 0.00001). Additional calcium scoring led to an increase in DLP (p = 0.15) and DSB levels (p = 0.04). DSB levels normalised to the DLP showed a significant correlation to the attenuation of the blood (rho = 0.53, p = 0.01) and a negative correlation to the body mass index of the patients (rho = -0.37, p = 0.06). CONCLUSION: gamma-H2AX immunofluorescence microscopy allows one to determine dose-related effects on x-ray-induced DSB levels and to consider individual factors which cannot be monitored by physical dose measurements. |
Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Radiation Biology and DNA Repair ?? fb10_zoologie ?? 10 Fachbereich Biologie |
Hinterlegungsdatum: | 07 Sep 2010 13:32 |
Letzte Änderung: | 05 Mär 2013 09:36 |
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