Ludewig, Uwe (2004)
Electroneutral ammonium transport by basolateral rhesus B glycoprotein.
In: The Journal of physiology, 559 (Pt 3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The liver and kidney are important tissues for ammonium (NH4+/NH3) metabolism and excretion. The rhesus B glycoprotein (RhBG) is a membrane protein expressed in liver and kidney with similarity to NH4+ transporters found in microorganisms, plants and animals. In the kidney, RhBG is predominantly localized to basolateral membranes of distal tubule epithelia, including connecting tubules and collecting ducts. These epithelia display mainly electroneutral ammonium transport, in contrast to other tubular sites, where net NH4+ transport occurs. In accordance with its localization, human RhBG mediates saturable, electroneutral transport of the ammonium analogue methylammonium when heterologously expressed in Xenopus oocytes. Uptake of methylammonium saturates with a Km = 2.6 mm. Methylammonium uptake is inhibited by ammonium and this inhibition saturates with a Ki approximately 3 mm. Electric current measurements and intracellular pHi determinations suggest that RhBG acts as an electroneutral NH4+ -H+ exchanger.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2004 |
| Autor(en): | Ludewig, Uwe |
| Art des Eintrags: | Bibliographie |
| Titel: | Electroneutral ammonium transport by basolateral rhesus B glycoprotein. |
| Sprache: | Englisch |
| Publikationsjahr: | 2004 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The Journal of physiology |
| Jahrgang/Volume einer Zeitschrift: | 559 |
| (Heft-)Nummer: | Pt 3 |
| Kurzbeschreibung (Abstract): | The liver and kidney are important tissues for ammonium (NH4+/NH3) metabolism and excretion. The rhesus B glycoprotein (RhBG) is a membrane protein expressed in liver and kidney with similarity to NH4+ transporters found in microorganisms, plants and animals. In the kidney, RhBG is predominantly localized to basolateral membranes of distal tubule epithelia, including connecting tubules and collecting ducts. These epithelia display mainly electroneutral ammonium transport, in contrast to other tubular sites, where net NH4+ transport occurs. In accordance with its localization, human RhBG mediates saturable, electroneutral transport of the ammonium analogue methylammonium when heterologously expressed in Xenopus oocytes. Uptake of methylammonium saturates with a Km = 2.6 mm. Methylammonium uptake is inhibited by ammonium and this inhibition saturates with a Ki approximately 3 mm. Electric current measurements and intracellular pHi determinations suggest that RhBG acts as an electroneutral NH4+ -H+ exchanger. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Pflanzenernährung und Biomasse ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 16 Mär 2010 15:07 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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