Dynowski, Marek ; Mayer, Maria ; Moran, Oscar ; Ludewig, Uwe (2008)
Molecular determinants of ammonia and urea conductance in plant aquaporin homologs.
In: FEBS letters, 582 (16)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2;1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH(3), and a residue without direct contact to the pore lumen was critical for conduction in the mutants.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2008 |
| Autor(en): | Dynowski, Marek ; Mayer, Maria ; Moran, Oscar ; Ludewig, Uwe |
| Art des Eintrags: | Bibliographie |
| Titel: | Molecular determinants of ammonia and urea conductance in plant aquaporin homologs. |
| Sprache: | Englisch |
| Publikationsjahr: | 2008 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 582 |
| (Heft-)Nummer: | 16 |
| Kurzbeschreibung (Abstract): | Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2;1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH(3), and a residue without direct contact to the pore lumen was critical for conduction in the mutants. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Pflanzenernährung und Biomasse ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 16 Mär 2010 13:12 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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