Kirchhofer, A. ; Helma, J. ; Schmidthals, K. ; Frauer, C. ; Cui, S. ; Karcher, A. ; Pellis, M. ; Muyldermans, S. ; Casas-Delucchi, C. S. ; Cardoso, M. Cristina ; Leonhardt, H. ; Hopfner, K. P. ; Rothbauer, U. (2010)
Modulation of protein properties in living cells using nanobodies.
In: Nature structural & molecular biology, 17 (1)
doi: 10.1038/nsmb.1727
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). One nanobody could reversibly reduce GFP fluorescence by a factor of 5, whereas its displacement by a second nanobody caused an increase by a factor of 10. Structural analysis of GFP-nanobody complexes revealed that the two nanobodies induce subtle opposing changes in the chromophore environment, leading to altered absorption properties. Unlike conventional antibodies, the small, stable nanobodies are functional in living cells. Nanobody-induced changes were detected by ratio imaging and used to monitor protein expression and subcellular localization as well as translocation events such as the tamoxifen-induced nuclear localization of estrogen receptor. This work demonstrates that protein conformations can be manipulated and studied with nanobodies in living cells.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2010 |
| Autor(en): | Kirchhofer, A. ; Helma, J. ; Schmidthals, K. ; Frauer, C. ; Cui, S. ; Karcher, A. ; Pellis, M. ; Muyldermans, S. ; Casas-Delucchi, C. S. ; Cardoso, M. Cristina ; Leonhardt, H. ; Hopfner, K. P. ; Rothbauer, U. |
| Art des Eintrags: | Bibliographie |
| Titel: | Modulation of protein properties in living cells using nanobodies. |
| Sprache: | Englisch |
| Publikationsjahr: | 2010 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nature structural & molecular biology |
| Jahrgang/Volume einer Zeitschrift: | 17 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1038/nsmb.1727 |
| URL / URN: | http://www.cardoso-lab.org/publications/Kirchhofer_2010.pdf |
| Kurzbeschreibung (Abstract): | Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). One nanobody could reversibly reduce GFP fluorescence by a factor of 5, whereas its displacement by a second nanobody caused an increase by a factor of 10. Structural analysis of GFP-nanobody complexes revealed that the two nanobodies induce subtle opposing changes in the chromophore environment, leading to altered absorption properties. Unlike conventional antibodies, the small, stable nanobodies are functional in living cells. Nanobody-induced changes were detected by ratio imaging and used to monitor protein expression and subcellular localization as well as translocation events such as the tamoxifen-induced nuclear localization of estrogen receptor. This work demonstrates that protein conformations can be manipulated and studied with nanobodies in living cells. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 06 Mär 2010 16:27 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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| Suche nach Titel in: | TUfind oder in Google |
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Modulation of protein properties in living cells using nanobodies. (deposited 05 Mär 2010 14:54)
- Modulation of protein properties in living cells using nanobodies. (deposited 06 Mär 2010 16:27) [Gegenwärtig angezeigt]
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